缺氧缺血性脑病新生鼠血8-异前列腺素F_(2a)变化及N-乙酰半胱氨酸的干预效果

Level of Blood 8-iso-Prostaglandin F_(2a) and the Intervention Effect of N-acetylcysteine on Neonatal Rats with Hypoxic-Ischemic Encephalopathy

目的探讨建立缺氧缺血性脑病(HIE)大鼠模型后不同时间段血8-异前列腺素F2a(8-iso-PGF2a)变化的意义,以及新型抗氧化剂N-乙酰半胱氨酸(NAC)对其干预效果。方法7日龄SD新生大鼠98只随机分为干预组、手术组和假手术组。干预组予NAC 0.1 mg/(g.d),手术组给予等体积生理盐水,假手术组不做相应处理。3组分别于建模后30 min,1、3、72、1 d断颈取血,采用EIA法检测血清8-iso-PGF2a含量。结果建模后30 min,手术组、干预组和假手术组血清8-iso-PGF2a水平分别为(168.7±24.2)(、120.9±23.4)、(50.0±8.8)ng/L,3组间有显著性差异(P均<0.05);24 h后,手术组、干预组大鼠8-iso-PGF2a水平逐渐降至假手术组水平,两组无差异。结论8-iso-PGF2a可作为HIE病情及预后的指标之一。NAC能有效地降低血HIE新生大鼠血中8-iso-PGF2a水平,对防治HIE有一定效果,可以考虑将其应用于临床。

Objective To explore the changes of blood 8-iso-Prostaglandin F2a（8-iso-PGF2a）content in different time after building-model and study its significance, and study the intervention effect of N-acetylcysteine（NAC） on it, Methods Seven-day-old Sprague-Dawley （SD） rats were randomly divided into 3 groups： intervention,operation and false operation groups, rats in intervention group were administered with NAC 0.1mg/（g·d）, Rats in operation group were given an equal volume of normal saline, and rats in false group operation had no administration. The blood was obtained to determine serum of 8-iso-PGF2a content in 30 minutes,on the first, third,seventh and twenty first day after the building-model, respectively. EIA assay method was used to determaine serum 8-iso-PGF2a content. Results Serunl 8-iso-PGF2a concentration in rats from operation group, intervention and false operation groups were（168.7±24.2）, （ 120.9±23.4） and （ 50.0± 8.8） ng/L within 30 minutes after the building-model, respectively. There were a remarkable difference betwecn serum 8 iso-PGF2a concentration in three groups（P〈0.05）. Serum 8-iso-PGF2a level in rats from operation and intervention groups decreased to the level in rats from false operation group 24 hours after the building model, and no statistical diffe-rence exited in these groups （P〉0.05）, Conclusions The concentration of serum 8-iso-PGF2a of rats rises remarkably early after building-model, and the peak concentration appears at 30 minutes after the building-model. NAC can effectively decrease serum 8-iso-PGF2a content, which suggest that there is large value of clinical administration.