衰老的分子机理研究

StudyontheMolecularMechanismofAging

研究年龄对大鼠不同组织（脑、心和肝）内源性多胺与ｃＡＰＭ含量的影响，测定了不同年龄大鼠脑组织鸟氨酸脱羧酶（ＯＤＣ）的活性，分析鼠脑Ｂ型革胺氧化酶（ＭＡＯ－Ｂ）的活性，作为进一步支持多胺与衰老是否相关的论据。实验表明，老年或幼年大鼠的多胺和ｃＡＭＰ的含量以及ｏＤＣ的活性都明显降低，而ＭＡＯ－Ｂ的活性则随年龄增加而增加。实验结果证明，代谢调节物（多胺和ｃＡＭＰ）的含量如同ＭＡＯ－Ｂ一样与衰老相关。它们含量的改变可能是衰老分子基础。

Inthepresentstudy,Weinvestigatedtheeffectofageonthecontentsofen-dogenouspolyaminesandcAMPindifferentialtissue(brain,heartandliver)ofrats.AnddeterminedornithineDecarboxylase(ODC)activityforbrainofratsatvariousage.AndanalvsedtheMonoamineOxidase-B(MAO-B)activityinratbrain,asapointupanargu-mentfordeterminewhetherthecorrelationbettwentheagingandpolyamine.Theexperientsasshowedthecontentsofpolyamine,cAMPandODCactivitywassignificantdecreaseinoldorpostnatalrats.AndtheMAO-Bactivitywasprogressiveincrease,withincreasingage(Tab1、2、3).ourexperimentresultevidencethatthecontentsofmetabolismmodulates(polyamineandcAMP)asSimilaritytotheMAO-B,withcorrelatedtotheaging.Itmaybepossibleamolecularbasisofaging.Therefore,thatpolyaminelevelscouldserveasatooltomonitoragingandantiagingtreatingeroutologyorgerocmia.