摘要
报道了荧光试剂9-(3-氨基苯胺基)吖啶(简称:m-APAA)的合成,产品经多次重结晶提纯,用IR1、HNMR和元素分析确证了结构。研究了该物质与DNA作用的荧光光谱,随着DNA的加入观察到418nm(λex=256nm)处的荧光峰猝灭,且其猝灭程度与DNA的浓度成正比,据此拟定了测定DNA的分析方法。实验了最佳条件,干扰实验,确定了反应机理。该方法简便、快速、灵敏度高。
A new fluorescent reagent 9-(3-aminoanilino)acridine(m-APAA)was prepared and the product was purified by several times by recrystallition. Its constitution was confirmed by IR, 1 HNMR and elemental analysis. The fluorescence spectra of m-APAA with Deoxyribonucleic acid (DNA) was studied. It was shown that DNA has ability to quench the m-APAA fluorescence in 418 nm(λex=256 nm) ,and the quenched intensity of fluorescence is proportional to the concentration DNA. This method had the advantages of high sensitivity,good selectivity and simplicity.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2005年第4期468-470,共3页
Chemical Research and Application