9-(3-氨基苯胺基)吖啶与核酸作用的荧光光谱及其分析应用

Study and applications of 9-(3-aminoanilino)acridine with DNA fluorescence spectra

报道了荧光试剂9-(3-氨基苯胺基)吖啶(简称:m-APAA)的合成,产品经多次重结晶提纯,用IR1、HNMR和元素分析确证了结构。研究了该物质与DNA作用的荧光光谱,随着DNA的加入观察到418nm(λex=256nm)处的荧光峰猝灭,且其猝灭程度与DNA的浓度成正比,据此拟定了测定DNA的分析方法。实验了最佳条件,干扰实验,确定了反应机理。该方法简便、快速、灵敏度高。

A new fluorescent reagent 9-（3-aminoanilino）acridine（m-APAA）was prepared and the product was purified by several times by recrystallition. Its constitution was confirmed by IR, 1 HNMR and elemental analysis. The fluorescence spectra of m-APAA with Deoxyribonucleic acid （DNA） was studied. It was shown that DNA has ability to quench the m-APAA fluorescence in 418 nm（λex=256 nm） ,and the quenched intensity of fluorescence is proportional to the concentration DNA. This method had the advantages of high sensitivity,good selectivity and simplicity.