摘要
luxR基因是革兰氏阴性菌感应反应(QuorumSensing,QS)LuxR/LuxI环路中起重要作用的基因.从水稻白叶枯病菌中分离到一个luxR同源基因,命名为xooR.xooR全长765bp,编码一个由254个氨基酸残基组成的转录因子,分子质量和等电点分别为28.3ku和8.72.XooR蛋白的N-端15AA-171AA是一个Autoind-bind结构域,C-端191AA-245AA是一个HTH(helix-turn-helix)结合DNA结构域.利用原核表达载体pET-24a(+)和gfp基因构建融合蛋白表达系统,Westernblot分析表明XooR-GFP融合蛋白大小为54ku,xooR基因成功地在E.coliBL21中实现了表达.
LuxR homologues are the main component of LuxR/LuxI circus in Quorum Sensing (QS) of Gram-negative bacteria. A luxR homologue was cloned from Xanthomonas. oryzae pv. oryzae, designated xooR. XooR is a transcriptional factor,its full-length spans 765 bp and encoded a putative protein of 254 amino acids, molecular weight and isolectric point are 28.3 ku and 8.72, respectively. The N-terminus froml5 amino acid to 171 amino acid of XooR is autoind-bing domain and the C-terminus 191 amino acid to 245 amino acid is helix-turn-helix DNA-binding domain. The prokaryotic expression vector pET-24a(+) was used to construct the fusion protein expression system. The vector was subcloned a gfp gene as a marker protein. Western blotting indicated that the XooR-GFP fusion protein is 54 ku. xooR gene was successful expressed as an XooR-GFP fusion protein in E. coli BL21 in vitro.
出处
《复旦学报(自然科学版)》
CAS
CSCD
北大核心
2005年第4期540-543,共4页
Journal of Fudan University:Natural Science
基金
Mcknight基金(14001404)
云南省自然科学基金重点资助项目(200C0010Z)
关键词
水稻白叶枯病菌
感应反应
xooR
表达
Xanthomonas orvzae pv. oryzae
Quorum Sensing
xooR
expression