丙型肝炎病毒E2基因在转基因番茄植株中的表达

Expression of Hepatitis C Virus E2 in Transgenic Tomato Plants

以T-E1E2为模板扩增得到丙型肝炎病毒包膜蛋白基因E2,构建该基因的植物表达载体p35s-E2,通过农杆菌介导的叶盘法转化番茄子叶.转基因番茄植株叶片总DNA的PCR、Southernblot检测结果表明,E2基因已整合进了转基因番茄植株基因组中;RT-PCR,Westernblot分析证实E2基因在转基因番茄植株叶片中表达.

Hepatitis C virus envelope protein gene E2 was amplified from the template T-E1 E2 and cloned into a modified plant expression vector to generate a new vector designated p35S-E2. Then Agrobacterium tumefaciens EHA105 containing p35S-E2 were used for leaf disk transformation in tomato. PCR and Southern blot were used to confirm the integration of the E2 gene in the transgenic plant genome. RT-PCR and Western blot analysis showed that the E2 gene was expressed in the transgenic tomato leaf.