东阿阿胶对体外培养的癌症放疗病人外周血淋巴细胞的影响

Effect of donkey-hide gelatin on the cultured peripheral blood lymphocytes of cancer patients treated by radio-therapy in vitro

目的观察东阿阿胶对体外培养的癌症放疗病人外周血淋巴细胞的影响。方法SD大鼠东阿阿胶灌胃给药3d后取血制备含药血清;取放疗病人外周血,经淋巴细胞分离液分离出淋巴细胞,接种于96孔培养板,东阿阿胶含药血清按分组剂量加入培养孔中,阳性对照为胸腺肽,37℃,5%CO2培养一定时间后,上流式细胞仪测定增殖率、T淋巴细胞活化抗原的表达、淋巴细胞表型及Th1/Th2细胞的比例。结果与对照组相比,不同剂量含东阿阿胶血清均能显著促进丝裂原诱导的淋巴细胞增殖,促进淋巴细胞活化,提高CD3+(T细胞)、CD3-CD56+CD16+(NK细胞)的比例和CD3+CD4+/CD3+CD8+比值,增加Th1,降低Th2。结论东阿阿胶能解除或减轻肿瘤和放疗对免疫系统产生的抑制作用,有助于免疫细胞对肿瘤的应答。

OBJECTIVE To investigate the effect of donkey-hide gelatin （DHG） on the cultured peripheral blood lymphocytes of cancer patients treated by radio-therapy in vitro. METHODS SD rats were given DHG orally for three days and then withdrawn the blood to isolate the drug-involved serum（DS）. Heparinized peripheral blood was obtained from the cancer patients treated by radiotherapy. The peripheral blood lymphocytes were isolated on lymphocyte separation medium and placed in 96-well culture plate. The DS was placed in each well according to dosage groups（ the positive-control group was placed with thymus peptide）. The cells in culture plate were cultured at 37℃ in 5% CO2 air for some time and then used in detecting cellular proliferation, expression of T lymphocyte activation antigen, lymphocyte immunophenotype and the ratio of Th1/Th2 cells. RESULTS Compared with the control group, the DS of different doses could improve the lymphocyte proliferation induced by mitogen and lymphocyte activation, increase the proportion of CD3 ^＋ （ T cells） and CD3^＋ CD56 ^＋ CD16^＋ （ NK cells） and the ratio of CD3^＋ CD4 ^＋/ CD3^ ＋ CD8^＋ , raise the ratio of Th1/Th2 cells. CONCLUSION DHG can get rid of or relieve the immune system depression induced by tumor and radio-therapy and conduce to immunocyte response to tumor.