摘要
目的研究石斛中石斛多糖的提取工艺。方法采用正交试验法进行优选,紫外分光光度法测定多糖含量。结果粉碎度、提取次数和加水量因素对石斛中石斛多糖的提取有显著性影响。结论石斛中石斛多糖的最佳提取工艺为将药材粉碎至粗粉,加水20倍量,提取4次,每次8h。
OBJECTIVE To study the extraction process of polysaeeharide from Caulis Dendrobii. METHOD The optimum extraction process was selected with the orthogonal design. The content of polysaccharide was determined by UV-spectrophotometry. RESULTS After breaking,the times of extracting and the amount of water had marked influence on the extraction of polysaccharide from Caulis Dendrobii. CONCLUSION The optimum extraction process of polysaccharide from Caulis Dendrobii was as following： grind Caulis Dendrobii to rough grains,adding 20 times water,extracting 4 times and 8 hours each time.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2005年第4期293-294,共2页
Chinese Journal of Modern Applied Pharmacy
关键词
石斛
正交试验
石斛多糖
紫外分光光度法
Caulis Dendrobii
orthogonal design
polysaccharide
UV-spectrophotometry
