Oncolyn对半乳糖致衰老小鼠氧化损伤影响的实验研究

Study of effect of Oncolyn on oxidative damage caused by galactose in senescent mice

目的探讨Oncolyn对D半乳糖致衰老小鼠氧化损伤的缓解作用。方法昆明种小鼠随机分为对照组、衰老组和Oncolyn干预组;用D半乳糖颈部皮下注射42d复制衰老小鼠模型。Oncolyn组在小鼠注射半乳糖后22d,经口给入Oncolyn,共70d。实验结束后取脑组织、血液和心脏组织检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)、一氧化氮(NO)、一氧化氮合酶(NOS)、丙二醛(MDA)、单胺氧化酶(MAO)、Na+K+ATP酶和Ca2+ATP酶的水平,以及海马和大脑皮层中Caspase3的表达情况。结果衰老组小鼠血液中SOD和GSHPx活性(143.19U/ml,105.81U)降低,NO、NOS(49.19mmol/L,25.29U/ml)的水平增加,与对照组(173.23U/ml,130.74U,39.20mmol/L,18.60U)比较差异有显著性。在心肌组织中Na+K+ATP酶和Ca2+ATP酶活性降低。脑组织中SOD、NO、NOS、GSHPx(24.57U/mgPro,1.28mmol/mgPro,0.46U/mgPro,4.79U/mgPro)水平均下降,而MDA和MAO水平(1.14mmol/mgPro,1.23U/mgPro)增加,同样Caspase3的蛋白表达无论在海马还是在大脑皮质中均增加。衰老小鼠经口给入Oncolyn后,血液中SOD和GSHPx活性均比衰老组增加,NO含量和NOS活性下降。心肌组织中Ca2+ATP酶的活性[0.31μmolPi/(mgPro·h)]比衰老组[0.22μmolPi/(mgPro·h)]增加。Oncolyn组小鼠脑组织中SOD、GSHPx活性增加,而NOS活性高于衰老组。另外脑组织中MDA和MAO的水平均低于衰老组。Caspase3在海马和大脑皮质的表达均下降。结论Oncolyn能提高机体氧化应激能力,对机体的衰老有一定的缓解作用。

Objective To investigate the neutralization effect of Oncolyn on oxidative damage in aging mice. To explore the effect of Oncolyn on the oxidative damage caused by D-galactose in aging mice. Method The Kun-ming mice were divided into three groups： control group, aging mice group and Oncolyn intervening group. The senescence model was made by injecting of D-galactose for 22 days, Oncolyn were administered by oral for 70 days from the 22nd day after injection of galactose. The levels of SOD, GSH-Px, NO, NOS, MDA, MAO in blood and brain tissue were detected, the activities of ATPase in myocardial tissues were also measured. Additionally, the expression of Caspase-3 in pallium and hippecampus was measured too, using immunohistochemical technique. Result Compared with the control group, the activities of SOD and GSH-Px in blood and brain tissue were all lowered in senescent mice, the levels of NO and NOS were higher in blood and lowered in brain tissue （P〈0.05）, and the levels of MDA and MAO, the expression of Caspase-3 in senescent mice rose than that in control group. In Oncolyn group, the activities of SOD and GSH-Px in blood were 190.31 U/ml and 163.15 U/ml respectively, were higher than that of aging group （were 143.19 U/ml and 105.81 U respectively, P〈0.05）, the change trends of SOD and GSH-Px in brain tissue were the same like that in blood, but the activity of NOS was higher in brain tissue, not lowered like that in blood. Additionally, the levels of MDA and MAO and the expression of Caspase-3 also were lower both in pallium and hippocampus of the mice from Oncolyn group compared with aging group. Conclusion Oncolyn could enhance the ability of anti-oxidation, thereby could remit the process of aging.