血清抗AT_1、α_1、β_1和M_2受体自身抗体与慢性肾功能不全的相关研究

Analysis of the autoantibodies against AT_1,α_1,β_1 and M_2 receptor in chronic with renal failure

目的:探讨抗血管紧张素Ⅱ受体1型(AT1-受体)、α1-肾上腺素受体(α1-受体)、M2胆碱能受体(M2-受体)、β1肾上腺素受体(β1-受体)自身抗体是否与慢性肾功能不全发病有关。方法:以合成的受体多肽片段为抗原,应用酶联免疫吸附测定(ELISA)技术检测。结果:慢性肾炎并肾功能不全组抗AT1、α1、β1和M2受体抗体阳性率分别为46.96%、43.93%、50.00%、40.90%,高血压合并肾损害分别为46.40%、46.40%、67.90%、46.4%,明显高于高血压无肾损害组和正常对照组(P<0.01)。结论:抗G蛋白偶联型受体自身抗体可能与慢性肾功能不全发病有关。

Objective：To explore the role of the auto,antibodies against angiotensin Ⅱ type 1 receptor（AT1-reeeptor）,α1-adrenergic re-ceptor（α1-receptor）, M2-musearrinlc receptor（ M2-receptor）,β1-adrenergic receptor（β1-receptor） in the development of chronic renal failure. Methods： The epitopes of the second extracellular loop of AT1 receptor, α1 receptor, M2 receptor and β1 receptor were synthesized and used respectively to screen sere autoantibodies from patients with chronic glomertdonephritis with early stage renal failure, with hypertension with renal failure, hypertension without renal failure and healthy blood donors（control） by ELISA. Results： In chronic glomerulonephrifis with renal failure patients, the positive rates of the auto,antibodles against AT1-receptor, α1-receptor,β1-receptor, M2-receptor and were 46.96% ,43.93%,50.00% ,40.90% in patients with hypertension with renal failure The positive rates of the auto,antibodies against AT1-reeeptor（46.4% ）,α1-receptor（46.4% ）,β1-receptor（67.9% ）, M2-receptor（46.4% ） and in patients with hypertension with renal failure were higher than those of patients with hypertension without renal failure（ P 〈0.01 ）, and they were also higher than those of healthy donors（ P 〈 0.01 ） . Conclusion： Those autoantibodies against AT1,α1、β1,M2-receptor might play important roles in the pathogenesis of the with of chronic renal failure patients.