一种新型腹膜纤维化大鼠模型的构建

Establishment of a new type rat model of peritoneal fibrosis

目的构建一种新型无菌性腹膜透析腹膜纤维化大鼠模型。方法34只SD大鼠随机分为空白对照组、生理盐水组、高糖组、脂多糖组和红霉素组。5周后,进行2h腹膜平衡试验(PET),检测腹膜功能;测定腹膜组织中羟脯氨酸含量;观察腹膜厚度、血管数及其它组织学改变;用免疫组化法测定腹膜组织纤连蛋白(FN)表达率。结果高糖+乳糖酸红霉素可致腹膜结构及功能类似无菌性腹膜透析相关性腹膜纤维化改变特征。结论高糖+乳糖酸红霉素可构建腹膜纤维化大鼠模型。

Objective To establish a new type of non-bacterial peritoneal fibrosis model in rats. Methods Thirty-four SD rats were divided into five groups： group1 （control）, group 2 （normal saline）, group 3 （4.25% dextrose solution）, group 4 （4.25% dextrose solution with lipopolysaccharide）, group 5 （4.25% dextrose solution with lactobionate erythromycin）. Animals were sacrificed after 5 weeks. A 2-hour peritoneal equilibration test （PET） was performed. Dialysate-toplasma urea ratio （D/Purea）, glucose reabsorption （D2/D0）, net ultrafiltration （UF） volume, the level of hydroxyproline and fibronectin were determined. Peritoneal membrane histology was evaluated by light microscopy. Results The D2/D0 ratio and net ultralrdtration volume in group 5 were significantly lower than groups 1 and 2 （P 〈 0.05）. The D/Purea ratio in group 5 was significantly higher than groups 1, 2 and 3 （P 〈 0.05）. The levels of hydroxyproline and fibronectin in group 5 were significantly higher than groups 1 and 2 （P 〈 0.05）. The thickness of the peritoneal membrane in group 5 was significantly greater than that in groups 1, 2 and 3 （P 〈 0.05）. The quantity of peritoneal vessels in group 5 was significantly greater than that in groups 1 and 2 （P 〈 0.05）. Conclusion A new type of non-bacterial peritoneal fibrosis model in rats could be established by 4.25% dextrose solution with lactobionate erythromvcin.