缬沙坦对大鼠梗死后心肌TNF-α及胶原表达的影响

Effect of valsartan on expression of myocardial TNF-α and collagen after myocardial infarction

目的:本研究欲通过观察缬沙坦对大鼠急性心肌梗死后的心肌TNF-α及胶原表达的影响,探讨缬沙坦对心肌梗死后心室重构的作用。方法:建立大鼠急性心肌梗死模型。实验动物随机分为假手术组、对照组、心肌梗死加缬沙坦组(予以缬沙坦30mg·kg-1·d-1灌胃)。15天后将三组动物处死,心肌组织经HE染色作形态学观察,用免疫组化SP法检测心肌组织切片内的TNF-α表达变化,用苦味酸-天狼星红染色检测心肌胶原变化,应用SPSS10.0计算机软件包行统计学分析。结果:心肌组织HE染色:心肌梗死加缬沙坦组和对照组可见心肌纤维溶解断裂、炎症细胞浸润等改变;而且心肌梗死加缬沙坦组较对照组TNF-α及心肌胶原表达量明显降低(p<0.01)。结论:缬沙坦可明显降低心肌梗死后心肌TNF-α及心肌胶原的表达,从而减轻了心室的重构。

Objective： To detect the effect of valsartan on expression of myocardial TNF-α and collagen after myocardial infarction.Methods： A acute myocardial infarction model of rat was established. All rats were randomly divided into three groups： sham operation group, control group and myocardial infarction treated with valsartan group （Fed with valsartan 30mg·kg^-1·d^-1）. All rats were sacrificedt after15 days. Myocardial tissue was stained with HE and pathological changes were observed. TNF-α was detected by immunohistochemical technique （SP method）. Myocardial collagen was detected by Picric acid-Sirius red. Software of SPSS 10.0 was used to analyze all data.Results： In the myocardial infarction treated with valsartan group and control group,myocardial fibre was dissolved and broken, infiltration of inflammatory cells was observed in necrosis zone. The expression of TNF-α,myocardial collagen in myocardial infarction treated with valsartan group was obviously lower than that in control group （ p〈0.01）.Conclusion： Valsartan can obviously attenuate the expression of TNF-α.myocardial collagen in myocardial tissue, and by this way reduces ventricular remodeling after myocardial infarction.