高血压病血管紧张素受体基因临床表型分析

Clinical genotypes of angiotensin Ⅱ type 1 receptor in primary hypertension

目的:探讨血管紧张素Ⅱ1型受体(AT1R)基因临床表型与高血压病和颈动脉硬化之间的关系.方法:应用聚合酶链反应结合限制性内切酶法检测AT1R基因的AC基因和C1166等位基因在正常人和高血压病患者中的频率.结果:高血压病患者AT1R基因AC型频率和C1166等位基因频率均明显高于正常对照组(P＜0.05～0.01);高血压病患者AC基因型组颈动脉内膜中层厚度(IMT)和IMT/D比率均比AA基因型组明显增高(P＜0.05～0.01).相关分析发现,高血压发病与年龄、颈动脉IMT和AT1R基因型呈显著正相关(P＜0.05～0.01);AT1R基因型与收缩压、舒张压及颈动脉IMT呈显著正相关(P＜0.01～0.001).结论:AT1R基因多态性与高血压的发生、发展有关,AC基因型对颈动脉IMT增厚有重要影响,AT1R基因多态性与高血压病患者颈动脉硬化关系密切.

Objective： To investigate the relationship between clinical genotypes of angiotensin Ⅱ type 1 receptor （AT1R） and pathogenesis of carotid arteriosclerosis in primary hypertension. Methods： The frequency of AC genotype and C1166 allele were measured by polymerase chain reaction combined with restriction enzyme digestion in primary hypertensives and controls. Results： The AC genotype frequency and C1166 allele frequency of AT1R gene were higher in primary hypertension than those of the controls （P ＜ 0.05～0.01）. Hypertensive subjects with AC genotype had increased carotid artery intima-media thickness （IMT） and IMT/D ratio than those with AA genotype. The correlation analysis revealed that the primary hypertension was positively correlated to the age, carotid artery IMT and AT1R genotype. AT1R genotype was positively correlated to the systolic blood pressure （SBP）, diastolic blood pressure （DBP） and carotid artery IMT. Conclusions： AT1R gene polymorphism is associated with the onset and development of primary hypertension. AC genotype has great impact on the carotid artery IMT while AT1R gene polymorphism is closely associated with the carotid arteriosclerosis in primary hypertension.