口腔黏膜成纤维细胞与癌细胞相互作用的基因表达特性研究

The study of genes expression characteristics of oralnormal mucosa fibroblasts following coculture with tongue squamous cell carcinoma cell line

目的:探讨口腔癌细胞与成纤维细胞间信号传递的分子机制。方法:将口腔癌细胞与正常黏膜成纤维细胞(NMFs)共培养,采用半定量RT-PCR方法,测定细胞共培养前后6种基因表达改变。结果:共培养之后,除IGFBP1表达较共培养前减少外,Notch3、TFPI-2TIMP-3、ST3、Tenascin-C基因表达均增加。这些基因分属生长因子/细胞因子类、信号转导/膜受体类、蛋白酶/蛋白酶抑制剂类、细胞基质及相关蛋白类,与ECM降解、血管生成、细胞生长和生存有关。结论:口腔黏膜成纤维细胞与癌细胞相互作用产生促肿瘤发生发展的调控因子和抗肿瘤的调控因子,二者间可能存在着复杂的调控网络。

Objective： To study the molecular mechanism between oral epithelial tumor cells and neighboring fibroblast mediated by signaling. Methods： Semiquantiative RT-PCR experiment of six genes expression performed on mRNA isolated from oral cancer cells, normal mucosa fibroblast and coculture cells. Results： Gene expression of Notch3、TFPI-2 TIMP-3、ST3、 Tenascin-C were increased and IGFBP1 was decreased. These genes could be assorted in several classes： growth factors, signal transducers, proteasea/proteases inhibitors, extracellular matrix compents or associated protein related to matrix degradation, angiogenesis, cell growth and survival. Conclusion： These data point out to the potential complex regulation of these families of proteins and raise the question concerning the global pro-or antitumoral consequences of these heterotypic interactions.