植物不同种属间共用微卫星引物的研究

Micro-satellite Primers Shared by Different Plant Species and Genera

微卫星标记是近年来发展起来的建立在PCR基础上的第二代分子标记,具有分布均匀、多态性高、共显性、选择中性等优点,已用于品种鉴定、构建连锁图谱、构建物理图谱、遗传多样性、居群和进化等方面的研究.由于来源于数据库的微卫星序列不能代表整个基因组微卫星的分布情况,而且数据库中的DNA序列仅局限于一些模式植物及经济作物.同时,通过基因组文库筛选获得微卫星序列既耗时又耗资.最近有很多研究表明,从某种植物来的微卫星引物可用于其近缘物种的扩增.本文概括介绍了植物不同种属间共用微卫星引物的研究情况及意义.

Micro-satellite markers are the second-generation molecular markers, which have been developed in recent years and are characterized by such favorable characteristics as even distribution, high polymorphism, co-dominance and selective neutrality, and used in the studies concerned with variety evaluation, linkage-map construction and physical-map construction, genetic diversity, population and evolution. Micro-satellite sequences from data banks cannot represent the micro-satellite distribution of a whole genome and DNA sequences from data bases only limited to some modeled plants and cash crops. Furthermore, it is time-consuming and fund-costing to screen micro-satellite sequences by means of genomic libraries. Many recent researches have denoted that the micro-satellite primers from a species can be used for the amplification for its genetically close species. The paper deals with the researches and importance of micro-satellite primers shared by different plant species and genera.