同系与异系移植MMP_s、TIMP_s表达的比较实验研究

Investigation of the expressions of MMP_s and TIMP_s in rat transplantation arteriosclerosis model of Chronic Rejection

目的:动态观察MMPs、TIMPs在移植动脉中的表达规律,探讨其在移植动脉硬化中的作用。方法:将Wistar大鼠120只、SD大鼠40只随机分为10组,异系(SDWistar)移植组和同系(WistarWistar)移植组各5组,分别于术后3d和1、2、4、8w采取。形态学观察、免疫组织化学SP染色法测定移植段腹主动脉内MMP1、TIMP1,3的表达、Northern杂交定量分析MMP2表达,并加以比较。结果:①术后2～8w异系移植组移植段腹主动脉内膜厚度较同系移植组显著增生(P<0.05)。②同系移植组MMP1,2和TIMP1较低,峰值均出现于术后2～4w;异系移植组表达显著增加,MMP1,2峰值出现于术后第2w,TIMP1峰值出现于术后第4w。③TIMP3在各组中表达均较低,仅见于细胞外基质中。结论:移植时慢性排斥反应过程中MMPs/TIMPs表达时象及量效的不平衡导致了细胞外基质合成与降解的失衡,是移植物的动脉硬化发生的重要原因。

Objcetive ： To dynamic observe the rule of the expressions of MMPs and TIMPs on the transplanted artery, Which is for discussing these functions of transplanted arteriosclerosis. Methods：The expressions of MMPs and TIMPs in the tissues of transplanted abdominal aorta were mensurated by morphology, immunohistochemical SP staining method and the expression of MMp-2 was detected by Northern blot quantltatlve analysis in abdominal aortic orthotopic transplantation modal. Results ： The thickness of tunica intima of the allografts was much evidently thicker than isografts 2 Weeks after transplantation（P 〈 0. 05）. The expresses of MMPs and TIMPs In the isografts came to the highest on 14th day and then declined.But the expressions of MMPs and TIMPs In the allografts were observably higher tgan the former,and the beak of the expression of TIMP-1 was prolonged to 4th week. TIMP-3 Kept lowly expressed In every groups. Conclusion ： The unbalance of expressions of MMPs/TIMPs leading to the turbulence Of synthesization and degradation ECM,is an important reason for the process of chronic rejection.