环孢霉素A预处理对大鼠移植肝脏影响的实验研究

Cyclosporine A preconditioning on liver preservation and transplantation in rat

目的:研究环孢霉素A(CsA)预处理保存大鼠肝脏对原位移植后的植肝功能影响。方法:将大鼠随机分为A组(对照组,HTK液保存组)和B组(实验组,HTK液+CsA保存组),两组均保存12h后行原位肝移植,分别于供肝保存0h、12h及植术后7d检测各组血清谷丙转氨酶(AST),谷草转氨酶(AST),乳酸脱氢酶(LDH)浓度及各时间段线粒体呼吸功能RCR及P/O值。通过HE染色及原位凋亡染色观察各组肝脏组织细胞形态学改变及凋亡情况。结果:B组血清ALT、AST、LDH活性于保存12h显著低于A组,B组各时间段RCR及P/O值于保存12h,移植术后0.5h及术后7d均高于A组。细胞形态学检查B组移植肝组织细胞结构改变轻微,凋亡指数于保存12h,术后0.5h均低于A组。结论:CsA预处理供肝主要通过保护线粒体功能减轻冷保存期和再灌注对大鼠供肝的损伤及抑制肝细胞的凋亡,使大鼠原位肝移植后存活率升高。

Objective ：To study the effect of Cyclosporine A preoonditionging on liver preservatian and transplantation in rat. Methods： Rats ware divided randomly into control group （group A,HTK solution preservation） and CsA precondioning group （group B,HTK solutian preservation ＋1μM CsA） .The livers of two groups were had been all preservated for 12 hours before operation of orthotopic liver transplantatian. The content of serum AST, ALT, LDG were all assayed during preservation in oh, 12h and 7d post liver transplantation,as well as determination of mitochondrial respiration function parameters （RCR and P/O）. The change of Cell morphology and apoptosis were also observed by TUNEL stain, and miclroscopse in two groups. Results： The content of serum ALT, AST and LDH in group B were less than in group A after presenvation for 12 hours. RCR and P/O parameters of mitochondrial respiration in group B on preservation for 12h, and for 30 min after operation were more than in group A.Cell morphology and apoptosis changed slingtly compared with group B. Conclusion ：CsA preconditioning can relieve injury of donated liver during cold preseration and reperfusion. it protects mitochondrial fuction of liver Cells and inlibits cell apoptosisa.