乙肝患者肝细胞刺激物质及其自身抗体与特异免疫复合物的测定研究

DETERMINATIONS OF HUMAN HEPATOCYTE STIMULATOR SUBSTANCE (HSS),ANTI-HSS AND HSS-IC SERA OF HEPATITIS B PATIENTS

自人胎肝中提取得肝细胞刺激物质（ＨＳＳ），分别建立了检测ＨＳＳ含量、抗ＨＳＳ自身抗体（ＨＳＳ－Ａｂ）、ＨＳＳ特异免疫复合物（ＨＳＳ－ＩＣ）的ＥＬＩＳＡ法。免疫组化研究证实，ＨＳＳ存在于人胎肝以及肝病患者的肝细胞浆内，但正常成人肝细胞中为阴性。正常人血清中ＨＳＳ含量为８３．０±３０．９ｇ／Ｌ未测出ＨＳＳ－Ａｂ和ＨＳＳ－ＩＣ；乙肝患者血清中ＨＳＳ含量、ＨＳＳ－Ａｂ和Ｈ％＋ＩＣ阳性率均显著升高。

After purifying hepatocyte stimulator substance (HSS) from human fetal liver, we estab-lished the ELISA techniques for detection of HSS,anti-HSS autoantibody (HSS-Ab ) and HSS specific im-mune complex(HSS-IC)in the sera. The immunohistochemistrv observation showed that HSS was dis-tributed in hepatocytoplasm of fetal liver and the liver from the patients with liver diseases. No HSS dis-tribution was found in normal human hepatocytes. The levels of HSS in normal sera were 83. O±30. 9g/L, but HSS- Ab and HSS-IC were not detected in these sujects. There were significant elevationsof HSS,HSS-Ab and HSS-IC in the sera of hepatitis B patients.