摘要
采用分光光度法测定了酵母细胞中辅酶Ⅰ(NAD+/NADH)的含量.结果表明:辅酶I浓度在一定范围内,吸光度随辅酶I浓度呈线性变化.建立了标准曲线和回归方程.方差检验证明该方法简便,快速,准确可靠,精密度高.
A spectrophotometric method of quantification of nicotinamide adenine dinucleotide was introduced.The result showed that the absorbency was variable with the different concentration of nicotinamide adenine dinucleotide. The standard curve and regression equation were established. Variance test proved the method was simple, reliable, rapid and exact.
出处
《郑州轻工业学院学报(自然科学版)》
CAS
2005年第2期20-22,共3页
Journal of Zhengzhou University of Light Industry:Natural Science
关键词
辅酶Ⅰ
分光光度法
酵母细胞
nicotinamide adenine dinucleotide
spectrophotometric method
yeast cell