三苯氧胺逆转卵巢癌细胞株多药耐药性的研究

Reversal of Multi-drug Resistance by Tamoxifen in Ovarian Carcinoma Cells

目的探讨三苯氧胺(TAM)逆转卵巢癌细胞株的耐药性和逆转机制。方法应用ATP-TCA法检测细胞株的耐药性及TAM的逆转效果,应用流式细胞仪检测TAM对罗丹明123(Rh123)在细胞内积聚和外排的影响,应用免疫荧光技术定量测定TAM对P糖蛋白表达的影响。结果TAM能增加阿霉素对耐药株的细胞毒性作用,能增加耐药株细胞内Rh123的积聚,减少其外排,而对P糖蛋白的表达没有影响。结论TAM能部分逆转卵巢癌细胞株的耐药性,其强度与维拉帕米相当,其作用机理是抑制P糖蛋白的功能,而对其表达水平没有影响。

Objective To investigate the reversal effect and carcinoma cell lines with multi-drug resistance（MDR）. Methods the mechanism of tamoxifen（TAM） in ovarian by ATP-TCA. The effects of TAM on Rh123 uptake and efflux were TAM on P-gp expression were analyzed quantitatively by immuno- The reversal efficacy of TAM were measured analyzed by flow cytometer. The effects of fluorescence technique. Results TAM could increase the toxicity of adriamycin （ADM） in A2780/ADM, but not in A2780. TAM could increase cellular rhodamine 123（Rh123） accumulation and decrease its efflux, but had no effect on the expression of P-gp. Conclusions TAM can reverse MDR in ovarian carcinoma cell lines partly, and is similar to verapamil（VPL）. TAM may act by blocking the function of P-gp and has no effect on the expression of P-gp.