兔脑射频热凝损伤的实验研究

Experimental study on rabbit cerebral damage induced by radiofrequency hyperthermia

目的研究在射频热凝过程中毁损参数变化对毁损直径的影响,同时初步探讨凋亡在脑损伤过程中的作用。方法家兔随机分组,在不同温度和时间条件下进行射频热凝毁损,术后14d取材,测量毁损直径。应用SPSS软件对资料进行统计处理。另外随机选取两组动物,进行射频毁损,毁损温度为80℃持续60s,术后24h处死动物,取材处理后观察病理变化。结果①当毁损时间恒定时,毁损直径(l)随毁损温度(T)的增加而明显增大,呈现直线上升趋势。l=-10.482+0.260T,r=0.982。②当毁损温度恒定时,毁损直径(l)与毁损时间(t)之间为对数曲线关系:l=-45.4355+13.3687t,r=0.957。③24h处死的兔脑组织均表现出由中心向外周的坏死、凋亡、正常的梯度变化。结论根据温度和时间可以调控射频损伤的范围。在射频毁损的过程中,凋亡参与了细胞损害。

Objective To study the pathological changes and parameters of the lesion caused by radiofrequency hyperthermia. Methods In the subcortical white matter of rabbit brains, the lesions were made at designed temperature and time. Rabbits were sacrificed after 24 h or 14 d. The damaged coagulation diameters were measured under magnification. The software of SPSS 11.5 for Windows was used to analysis the data. Tissue treated by radiofrequency hyperthermia in given different periods and temperatures were examined respectively. Results ①When the time of radiofrequency hyperthermia was invariableness, the relationship between the lesion＇ s diameter （ L ） and temperature （T） was analysed： L = - 10. 482 ＋ 0. 260 T, r = 0. 982. ②When temperature of radiofrequency hyperthermia was invariableness, logarithmic curve estimation analysis showed that the relationship between lesion＇s diameter （ l ） and the time of radiofrequency hyperthermia （ t ） was： l = - 45. 435 5 ＋ 13. 368 7 t, r = 0. 957。③Apoptosis was observed at the edge of lesion on 24 h. Conclusion The coagulated masses can be controlled based on parameters of the radiofrequency hyperthermia. Apoptosis is one of the mechanisms that causes neural damage.