红景天中药复方改善机体骨骼肌能量代谢效果研究

STUDY ON IMPROVING ENERGY METABOLISM OF SKELETAL MUSCLE WITH THE COMPOUND OF EXTRACTED RADIX ET RHIZOMA RHODIOLAE IN RAT DURING HYPOBARIC HYPOXIA

本文为提高高原移居人体劳动能力，研制了以红景天提取物为主，含刺五加、黄芪和丹参的中药复方（９２０４０２复方）。动物随机分为实验组（９２０４０２复方组）和对照组。在小鼠减压缺氧（模拟海拔高度１００００ｍ）游泳实验中，实验组游泳时间明显长于对照组，分别为７５．８±４．４和６２．６±４．４ｍｉｎ，延长约２１．１％（Ｐ＜０．０５）；实验组游泳时间大于６０ｍｉｎ的存活卒明显高于对照组，分别为５５．４％和３６．９％（Ｐ＜０．０５）。在大鼠减压缺氧（模拟海拔高度６０００ｍ）游泳实验中，实验组骨骼肌线粒体ＲＣＲ和ＡＤＰ／Ｏ明显高于对照组（Ｐ＜０．０１），并且ＡＴＰ含量也高于对照组（Ｐ＜０．０５）。结果提示，９２０４０２复方能明显提高缺氧机体运动能力，其机制可能与９２０４０２复方改善缺氧机体骨骼肌能量代谢有关。

AbstractIn order to improve human work capacity at altitude,the effects of a compound of Radix et Rhizoma Rhodiolae(920402 compound) on exercise performance(survial time,ST) and energy metabolism of skeletal muscle of swimming-rat during hypobaric hypoxia were investigated.The mice were randomly divided into test(T)group(receiving 920402 compound)and con-trol(C)group.The animals were forced to swim in the simulated altitude of 10000m and 6000m in a hypobaric chamber, respectively.The results showed that the ST in T and C groups were 75.8±4.4min and 62.4 ±4.4 min,respectively (P<0.05),The survival rate of T group increased by 18.5%in control to that of C group(P<0.05)The respiratory control rate(RCR) and oxidative phosphorylation rate (ADP/O) of the skeletal musclemitochondria of T group were significantly higher than those of C group(P<0.01).The level of ATP in T group was increased markedly comparedwith that in C group(P>0.01).These results indicated that 920402 compoundmight increase the physical work capacity and improve energy metabo-lism of skeletal muscle at high altitude.KEY W0RDS:hypoxia, respiratory control rate, oxidative phosphorylation,physical work capacity