内脏伤害性刺激诱导的大鼠延髓内Fos表达

VISCERAL NOXIOUS STIMULATION INDUCED Fos EXPERESSION IN THE MEDULLA OBLONGATA OF THE RAT

用抗Ｆｏｓ蛋白和酪氨酸羟化酶（ＴＨ）的免疫组织化学方法，对大鼠两种内脏（胃肠道和心脏）伤害性刺激诱导的大鼠延髓Ｆｏｓ表达情况及其与儿茶酚胺能神经元的关系进行了观察。结果表明：Ｆｏｓ样免疫阳性神经元主要分布在延髓中段和尾段的孤束核、腹外侧区以及两者之间的网状结构内；在抗Ｆｏｓ和抗ＴＨ的双重免疫染色切片上，见到许多Ｆｏｓ阳性神经元的胞浆同时呈ＴＨ样免疫反应阳性（Ｆｏｓ／ＴＨ双重阳性细胞），计数结果证明Ｆｏｓ／ＴＨ细胞占ＴＨ阳性细胞总数的５０％以上，提示延髓内脏带半数以上的儿茶酚胺神经元参与对内脏伤害性刺激的反应。

Byusing immunohistochemical staining for Fos and tyrosine hydroxylase(TH),the Fos expression and its relationship with catecholaminergic neurons were examined in the medulla oblongata of the rat following received visceral(gastointestinal or cardiac)noxious stimulation.The Fos-like immunoreactive(Fos-LI)neurons distributed mainly in the nucleus tractus solitarius(NTS),ventrolateral medulla(VLM)and the reticular formation(RF)between the NTS and VLM,In the sections processed for double staining of Fos and TH immunohistochemstry,numerous Fos-LI neurons showed also TH-like immunoreactivity in their cytoplasm(Fos/TH cells).Cell count showed that the Fos/TH neurons constituted more than 50%of TH-LI cells.The present study suggested that over 50%of the catecholaminergic neurons in the visceral zone of the medulla oblongata were involved in response to visceral noxious stimulation.