大鼠破骨细胞体外分离培养和鉴定

CHARACTERIZATION,ISOLATION AND CULTURE OF OSTEOCLASTS FROM NEONATAL RAT LONG BONES

采用机械分离方法,从新生大鼠长管骨分离破骨细胞获得成功。分离的破骨细胞具有如下特性:1.在体外迅速贴附于盖玻片或骨片;2.多核(一般为3～10个细胞核);3.伪足运动活跃,降钙素或降钙素基因相关肽可以抑制其伪足运动;4.酸性磷酸酶染色强阳性,酸性醋酸酯酶弱阳性,PAS中等阳性,5.细胞悬液与骨磨片共同培养,可以形成骨吸收陷窝。由此可见,本研究分离的细胞,符合破骨细胞的公认特性,故本法分离培养破骨细胞是可行的。

Osteoclasts were isolated mechanically from neonatal rat long bones and cultured on glass or devitalized human cortical bone slices. Both morphological and functional studies showed that the isolated cells were of typical osteoclasts, because; 1. they attached rapidly on the matrix and had pseudopodial activity when cultured on glass;2. they had multinuclei,most of them contained 3-10 nuclei;3. calci-tonin and clacitonin gene related peptide directly inhibited the cellular activity; 4. they showed high acid phosphatase activity; 5. resorption lacunae could be found when the cells cocultured with devitalized bone slices.