Survivin在T细胞中的表达规律

Express Rule of Survivin in T Cells

目的:观察survivin在T细胞中的表达规律。方法:免疫细胞化学染色方法观察淋巴组织中及抗原刺激后T细胞的survivin表达。用抗IL-2受体抗体抑制T细胞增殖,流式细胞术检测CD3、CD25、survivin的表达;建立小鼠移植物抗宿主反应(GVHD)模型,观察受者肝脏内T细胞浸润和survivin的表达。结果:淋巴组织及同种抗原、ConA刺激后培养的脾细胞可表达survivin。survivinI+细胞为T细胞,T淋巴母细胞可同时表达CD25和survivin。抗IL-2受体抗体抑制survivin表达。第4-12天,GVHR小鼠肝脏中可观察到T细胞浸润并表达survivin。结论:T细胞激活后可表达survivin,可作为激活T细胞的标志,其可将T细胞对同种抗原反应的过程分为激活和效应过程。

Objective： To investigate the expression rule of survivin in T cells. Methods： The expression of survivin in splenocytes stimulated with ConA or alloantigen was detected by immunohistochemical straining. Anti-IL-2 receptor antibodies were added to inhibit T cells proliferation. Flow cytometry was used to analyze CD3, CD25 and the survivin expressions. Splenocytes from C57BL/6（H-2b） mice were infused into F1 mice （BALB/c× C57BL/6） to develop GVHR model, and the expression of survivin was observed by immunohistochemical strain in vivo after alloantigen stimulation. Results： The expression of survivin was detected in the stimulated cells. Survivin＋ cells were CD3＋ T cells, and lymphoblasts could simultaneously express CD25 and survivin. Anti-IL-2 receptor antibodies inhibited the survivin expression. From day 4 to 12 after infusion, the alloantigen-activated T cells infiltrated in livers of GVHR mice and expressed survivin. Conclusion： As a marker of activated T cells, survivin can be detected in T cells after stimulation and divided the procedure of T cells response to alloantigen into activation and effect phase.