EDDF诱导产生与红细胞终末分化相关的HS_2特异结合蛋白

THE HS_2 SPECIFIC BINDING PROTEINS RELATED TO THE ERYTHROID TERMINAL DIFFERENTIATION INDUCED BY EDDF

以珠蛋白基因增强子为探针，研究从ＦＶＡ病毒诱导的小鼠脾脏中提取的ＥＤＤＦ诱导小鼠红白血病细胞（ＭＥＬ）及人红白血病细胞（Ｋ５６２）后的表达产物与ＨＳ_２特异结合的可能性。结果表明，经ＥＤＤＦ诱导后的ＭＥＬ及Ｋ５８２细胞核中均可检测到两个与ＨＳ２结合的蛋白，其分子量约为１２ｋＤ和１４ｋＤ。提示ＥＤＤＦ有可能通过诱导产生与ＨＳ２特异结合的反式调节因子而导致红系细胞分化。

Erythroid differentiation and denucleation factor(EDDF)can induce the mouse erythroleukemia cells(MEL)and human erythroleukemia cells(K562)to differentiate. In order to reveal its mechanism,we undertook an experiment to study whether EDDF can induce MEL and K562 cells to express the specific HS2 binding proteins by using digoxin labelled HS2 DNA fragment probe,The results demonstrated that two specific HS2 binding proteins with molecular weights 12kD and 14kD respectively did exist in MEL and Ko62 cell nuclear extract induced by EDDF,Thus EDDF may induce erythroid cells lines to differentiate probably by expression of trans-acting factors which bind HS2 specifically.