ACE活性和eNOS含量与妊高征相关性研究

The activity of angiotensis converting enzyme(ACE) and the exprssion of endothelial nitric oxide synthase( eNOS) : Relations to pregnancy-induced hypertension

目的:探讨胎盘组织一氧化氮合酶含量和血清中血管紧张素转化酶(ACE)活性改变在妊高征发生、发展中的作用。方法:选择轻、中、重度妊高征病人各10例为实验组,正常妊娠妇女30例为对照组,用马尿酸微量比色法检测血清中ACE活性,采用免疫组化ABC法分析妊高征胎盘组织中内皮型一氧化氮合酶(eNOS)含量的变化情况。结果:1妊高征组ACE活性明显高于对照组;2妊高征患者胎盘中eNOS含量显著低于正常足月孕者(P<0.05),且轻度妊高征患者胎盘eNOS含量显著高于中度及重度患者(P<0.05)。结论:血清ACE活性升高与妊高征的发生、发展密切相关。正常足月孕胎盘和妊高征患者胎盘绒毛血管内皮细胞中均存在eNOS抗原,妊高征患者胎盘中含量的减少与其发病有关。

Objective： To investigate the expression of placental endothehal nitric oxide synthase （eNOS） and the function of angiotensis converting enzyme（ACE）in the occurrence and development of pregnancy- induced hypertension （PIH）. Methods： The study group included 30 pregnant women diagnosed with PIH（10 mild, 10 moderate, 10 severe）. Thirty healthy pregnant women were selected as the control group. The serum was assayed for the activity of ACE by using color comparison method. The placentas eNOS were studied by innunohistochemistry （Avidin--Biotin--Complex method）. Results： ① The ACE activity of the study group was significantly higher than that of the control group. ②The expression of placenta eNOS decreased significantly in patients with PIH as compared with that of normal pregnancy （P〈 0.05 ）. The expression of placenta eNOS in mild PIH was higher than that of moderate or severe PIH （P 〈 0. 05,P 〈 O. 05 ） Conclusion： The elevation of serum ACE activity is related to the occurrence and development of PIH. There are eNOS antigens in placental tissues from PIH and normal pregnancy. They are all localized in placental villi and svncvtiotronhoblast cells. It is concluded that the decrease of the expression of eNOS placenta may be associated with the pathogenesis of PIH.