中耳用药对豚鼠平衡器影响的电镜观察

AN ELECTRON MICROSCOPIC OBSERVATION ON THE EFFECT OF ADMINISTRATION OF DRUGS IN THE MIDDLE EAR ON EQUILIBRATIVE ORGANS IN GUINEA PIGS

通过注入0.5%,1%,2%环丙沙星与庆大霉素溶液于豚鼠中耳(听泡)内,对内耳组织进行火棉胶切片及扫描电镜观察标本制作,借助光镜和扫描电镜观察平衡器的显微和超微结构的变化,并通过平衡功能的测试,比较环丙沙星与庆大霉素对平衡器的毒性作用,结果证明:环丙沙星对平衡器的毒性很低,功能与形态上都没有明显损伤.庆大霉素则使壶腹嵴毛细胞纤毛融合,缺失,壶胶帽消失,椭圆囊斑和球囊斑的毛细胞纤毛粘连,缺损,耳右变性,减少或消失.

0.5%, 1% and 2% solution of ciprofloxacin and gentamycin were administrated into the middle ears(bulla tympanic) of guinea pigs. All those animals were decapitated 7 days after administration. The pathololgical changes in microstructure and ultrastructure of equilibration organs were examined by light microscopy and scanning electron microscopy. The equilibration function was tested for the' evaluation of vestibulotoxicity of ciprofloxacin and gentamycin. The results indicated that ciprofloxacin had less vestibulotoxicity than gentamycin and caused no remarikable damage in function and morphology. However, the administration of gentamycin caused the fusion and lesion of the stereocilia of sensory hair cells, the disapparance of the cap of compullaris, the necrosis and adhesion of hair cells of the cilias of macula of the utrichi and sacculi, and the degeneration or disapparance of the otoconia.