大鼠膈腹膜间皮孔的电镜观察

AN ELECTRON MICROSCOPIC OBSERVATION ON MESOTHELIAL STOMATA OF DIAPHRAGMATIC PERITONEUM IN RAT

用扫描电镜和透射电镜观察了正常大鼠隔腹膜间皮,并观察了腹膜腔内注射中国墨汁和兔血液后大鼠膈腹膜间皮的变化以及腹膜腔和间皮下毛细淋巴管的关系.结果发现,膈腹膜的间皮分非陷窝区和陷窝区.正常鼠陷窝区无间皮孔,间皮细胞的深方为毛细淋巴管,二者间仅有少量的结缔组织分隔.腹膜腔内注射兔血液和墨汁后,间皮细胞间出现间皮孔,腹膜腔内的碳颗粒和红细胞等经间皮孔可进入毛细淋巴管腔.非陷窝区未见间皮孔和毛细淋巴管.

An observation was made with transmission and scanning electron microscopy on the peritoneal mesothelium of the diaphragm in normal rats and its changes following intraperitoneal injection of China ink and rabbit blood. The relationship between peritoneal cavity and submesothelial lymphatic capillarries of the diaphragmatic peritoneum were also investigated. The peritoneal mesothelium of the diaphragm in rat could be divided into lacunar and non-lacunar areas. In the lacunar area of the diaphragmatic peritoneum, the submesothelial lymphatic capillaries were found beneath mesothelial cells, but in the non-lacunar area, no submesothelial lymphatic capillaries were identified. The mesothelial stomata in the lacunar area were not observed in normal rat. Mesothelial cells of the diathragmatic peritoneum and submesothelial lymphatic capillaries in the lacunar area were separated by a thin layer of connective tissues. After intraperitoneal injection of rabbit blood and China ink, mesothelial stomata appeared between mesothelial cells in the lacunar area. Carbon particles and red blood cells entered the lumen of the lymphatics from the peritoneal cavity via mesothelial stomata. Mesothelial stomata were not found in the non-lacunar area.