一氧化氮合酶在缺氧胎鼠组织中的表达及当归保护机制的实验研究

NOS EXPRESSION IN HYPOXIA FETAL RATS AND THE PROTECTIVE EFFECTS OF CHINESE ANGELICA ON BONE MARROW,NERVOUS TISSUE AND CARTILAGINOUS TISSUE

目的探讨一氧化氮合酶(NitricOxideSynthase,NOS)在宫内缺氧状态下胎鼠骨髓、软骨、神经组织中的表达,以及中药当归注射液(Angelicainjection)对宫内缺氧组织的保护作用。方法孕龄19天的Wistar孕鼠随机分为对照组、缺氧组和当归组,分别给予尾静脉注射0.9%生理盐水、尾静脉注射0.9%生理盐水后建立缺氧模型及尾静脉注射25%的当归注射液后建立缺氧模型处理,行石蜡切片和免疫组化实验以及图像分析和统计分析。结果对照组组织中nNOS低表达,缺氧组和当归组中nNOS高表达;而后两组比较,当归组nNOS含量比缺氧组明显减少(P<0.05)。结论成功建立宫内胎鼠缺氧模型。nNOS激活产生适量NO对胚胎发育起着生理调节作用,其过量表达则可能是宫内缺氧时引起胚胎多种组织细胞,特别是神经组织损伤的重要机制之一。当归注射液下调nNOS表达活性,对宫内缺氧胎鼠有保护作用。

Objective： To investigate the expression of NOS and the protective effects of Chinese angelica injection on fetal rats＇ hypoxia tissue in uterus. Methods： 12 female pregnant Wistar rats were randomly divided into three groups：the control group（CG）, the hypoxia group（HG） and angelica injection group（AG）, and each group having 4 rats. The rats of AG were injected with 25% Chinese angelica injection （8ml/kg） through tail vein before they were in hypoxia state. Ten embryos of each group were made into paraffin sections. The expression of NOS was then determined by immtmohistoehemistry, and their average light - density disposed by image processing system was analyzed with statistical methods. Result： In bone marrow, nerve and cartilage tissues observed, the NOS - postive cells were young erythroblasts, nerve cells and chondroeyte, respectively. The immunohistochemical staining and the mean optical density of NOS was low in CG and high in HG and AG（ P 〈 0.05）. In AG, the value was significantly lower compared with that in HG（ P 〈 0.05）. Conclusion： The model of intra- uterine hypoxia fetal rats was established. Angelica injection might down- regulate nNOS expression to protect the hypoxia fetal rats.