大豆总异黄酮的提取及含量测定

The extraction and Separation of isoflavonoids from soybean and the quantification of the isoflavonoids

目的建立大豆总异黄酮的提取方法及含量测定方法。方法以大豆为原料通过乙醇浸泡提取,聚酰胺柱层析提取大豆总异黄酮。并用HPLC法测定大豆总异黄酮含量。结果提取物物大豆总异黄酮含量为52.26%。HPLC的条件为:C18柱,流动相为甲醇∶水∶乙酸=42∶57∶1,检测波长为260 nm。结论提取方法简单,测定方法简便、快捷,重现性好,可用于大豆总异黄酮的含量测定。

Objective Establishing the method of the extraction and separation of isoflavonoids from soybean and the method of quantitative assay. Method The isoflavonoids from soybean were extracted by alcohol and separated by the column of polyurethane. And the isoflavonoids from soybean were quantified by HPLC. Result The isoflavonoids contend was 52.26%. The HPLC condition ： Column is C18; eluant is methanol-water-acetate acid （42： 57：1 v/v） ; detecting wavelength is λmax 260 nm. Conclusion The extract method is simple and the quantitative method can be used in the quantitative isoflavonoids from soybean simplely, quickly and reliablely.