联合检测端粒酶和CK20诊断大肠癌外周血微转移的研究

Study of colorectal carcinoma micrometastases in peripheral blood by using cytokeratin 20 and telomerase activity

目的联合检测细胞角蛋白20(cytoker-atin20,CK20)表达和端粒酶的活性,初步探讨它们在检测大肠癌微转移中的意义。方法用RT-PCR法,检测52例大肠癌患者术前和术后不同时间外周血中的CK20的表达。用端粒重复片段扩增方法检测相应患者外周血中的端粒酶的活性。结果52例患者外周血中检出CK20表达及端粒酶活性检测阳性率按不同时间段分别为61·9%与57·7%(术前),69·1%与72·1%(术后24h),34·6%与30·9%(术后早期化疗结束后)。两者的阳性表达均与患者Duke’s分期存在显著性相关。结论端粒酶和CK20均可作为标志物来检测大肠癌患者外周血中微转移。

OBJECTIVE： To detect cytokeratin 20（CK20） mRNA, telomerase activity and their correlation with micrometastasis of colorectal carcinoma. METHODS： CK20 mRNA and telomerase activity in peripheral blood of 52 patients with colorectal carcinomas were detected at different time points before and after operation by RT-PCR and TRAP respectively. RESULTS： At different phases, the positive rates of CK20 mRNA and telomerase activity of those patients were 61.9% vs 57.7 （before operation）, 69.1% vs 72.1% （24 h after operation） and 34.6% vs 30.9% （after the short-term chemotherapy） respectively. CONCLUSION： CK20 mRNA and telomerase activity may be potential tumor markers for detecting micrometastasis and recurrence in peripheral blood.