乙醇脱氢酶2基因多态和饮酒习惯与肝癌易感性

Genetic polymorphisms of alcohol dehydrogenase-2 and alcohol drinking for the susceptibility of the primary hepatocellular carcinoma

目的:研究乙醇脱氢酶2(ADH2)基因多 态性和饮酒习惯与肝癌的易感性关系。方法:在 肝癌高发区江苏省泰兴市采用病例对照研究方 法,病例为确诊的原发性肝癌(207例)新发病例, 对照(207例)按1∶1配对,选取与病例同性别、年 龄相差不超过2岁,与病例居住同村或同一街道 的非肿瘤居民,调查研究对象的饮酒习惯等因素, 并采用聚合酶链反应 限制性片段长度多态性 (PCR PFLP)方法检测了他们的ADH2基因型。 结果:1)病例组及对照组中ADH2 1/ 1、 ADH2 1/ 2、ADH2 2/ 2的基因型分布频 度分别为10.14%、63.77%、26.09%及12.56%、 46.86%、40.58%。与携带ADH2 1/ 1基因型 者相比,无论是携带ADH2 2/ 2(OR=0.8, 95%CI=0.39～1.64)或ADH2 1/ 2(OR= 1.68,95%CI=0.86～3.32)基因型者,患肝癌的危 险性均无明显增加。2)携带ADH2 1/ 2或 ADH2 2/ 2基因型饮酒者与携带ADH2 1/ 1基因型的不饮者相比,患肝癌的风险差异 无统计学意义(OR=0.997,95%CI=0.296～ 3.354)。3)在调整了年龄、性别、HBsAg、肝硬化和 血吸虫病因素后,结合饮酒习惯将前者与后者相 比,若开始饮酒年龄<25岁、饮酒量>3kg/月、饮 酒年数>23年和饮酒总量(千克年)>3时,前者 患肝癌的危险性分别是后者的1.88(95%CI=0.53 ～6.70)、1.

OBJECTIVE： To investigate the relationship between alcohol dehydrogenase-2 （ADH2） genotypes and alcohol drinking for the susceptibility of primary hepatoeellular carcinoma （PHC）. METHODS： A case-control study including 207 cases of PHC and 207 controls matched with sex, age and residential area was carried out in Taixing City of Jiangsu Province, China. Blood .samples were collected and tested for ADH2 genotypes by PCR-RFLP method. RESULTS： 1） The frequency of ADH2＊ 1/＊ 1, ADH2 ＊ 1/＊ 2and ADH2 ＊ 2/＊ 2 genotypes were 10. 14%, 63.77% and 26. 09% in casesof PHC, and 12. 56%, 46. 86% and 40. 58% in the controls. Compared with individuals with ADH2 ＊ 1/＊ 1 genotype, the individuals with ADH2 ＊ 2/＊ 2（OR=0. 80, 95%CI=0. 39-1.64） and ADH2 ＊ 1/＊ 2（OR=1. 68,95%CI=0. 86-3. 32） genotypes were not at a high risk of developing PHC. 2） No significant differences were found between drinkers with ADH2 ＊ 1/＊ 2 or ADH2 ＊ 2/＊ 2 genotype and no-drinkers with ADH2 ＊ 1/＊ 1 genotype in the developing of PHC（OR= 0. 997, 95% CI= 0. 296-3. 354） . 3） Drinkers with ADH2＊ 1/＊ 2 or ADH2＊ 2/＊ 2 genotype and any of the following factors： age started drinking 〈25, the amount of alcohol consumption per month 〉3 kg, the duration of alcohol consumption history 〉23 years and cumulative amount of alcohol consumption 〉3 （kg ＊ years） were not at a significantly risk of developing PHC [adjusted OR = 1.88 （95%CI= 0. 53- 6. 70）, 1. 35 （95%CI= 0. 36- 5. 04）,1. 46 （95%CI=0. 41-5. 17） and1. 46（95%CI=0. 39-5. 47）, respectively] compared with those no-drinkers with ADH2 ＊ 1/＊ 1 genotype. CONCLUSIONS： ADH2 polymorphisms may have no important role in the developing of PHC.