急性重复缺氧暴露小鼠海马HIF-1α表达的变化(英文)

Expression alterations of hypoxia-inducible factor-1α subunit in the hippocampus of mice acutely and repeatedly exposed to hypoxia

目的检测小鼠脑海马组织中HIF-1α在急性重复缺氧条件下的变化,探讨缺氧预适应过程中HIF-1α活化对脑的保护作用。方法小鼠缺氧0次(HO),1次(H1),4次(H4)后取海马组织,应用RT-PCR技术、Western Blot、EMSA对HIF-1αmRNA、蛋白质含量及HIF-1DNA结合活性进行测定。结果与H0组相比,H1组HIF-1αmRNA显著升高,而H0和H4之间未见显著差异。蛋白质含量及HIF-1DNA结合活性在H0、H1、H4中依次增加。结论HIF-1α蛋白质的含量及HIF-1DNA结合活性增加可能参与缺氧预适应小鼠脑保护。

Objective To detect the effect of hypoxia preconditioning on HIF-1α expression in the hippocampus of mice during acute and repeated hypoxia exposures. Methods Mice were randomly divided into 3 groups and were exposed to hypoxia for 4 runs （ H4 group） , 1 run （ H1 group） and 0 run （ control group）. RT-PCR, Western blot and EMSA were used to examine the HIF-1α expression in the mouse hippocampus following exposure to hypoxia. Total mRNA,total protein and nuclear protein were extracted from the mouse hippocampus for RT-PCR, Western blot and EMSA respectively. Results HIF-1α mRNA levels were found to be increased in H1 and decreased in H4 （vs H1）. HIF-1α protein levels and HIF-1 DNA binding activities were increased in H1 and markedly increased in H4. Conclusion HIF-1 activation may be involved in the protection of the brain of hypoxia-preconditioned mice.