HPLC法测定二花三草合剂中绿原酸的含量研究

Determination of Chlorogenic acid in ErHuaSanCao Mixture With HPLC

目的:建立高效液相色谱法测定二花三草合剂中绿原酸含量的方法。方法:色谱条件采用Diamosil-C18柱,以乙腈-0.4%磷酸(18:82)为流动相;流速为0.8mL·min-1;检测波长为327nm。结果:绿原酸在0.0960μg^0.9600μg范围呈良好线性关系;其回归方程为Y=6773.972+2629723.174X(r=0.9999);平均回收率为98.89%;RSD为1.52%(n=6)。结论:本方法操作简单、准确,可用于测定二花三草合剂中绿原酸含量。

Objective： To establish a method to determine the content of Chlorogenic acid in ErHuaSanCao Mixture by HPLC.Method： Chromatography was carried out on Diamosil -C18column,using acetonitrile -0.4% phosphoric acid solution（ 18 ： 82） as mobile phase; The flow rate was 0.8 mL · min^-1 ;The detective wavelength was 327nm. Result： Chlorogenic acid showed a good linear relationship in the range of 0. 096 - 0. 960μg, the regression equation was Y = 6773. 972 ＋ 2629723. 174X （ r = 0.9999） ; The average recovery was 99.38% and RSD 1.57% （n = 6）. Conclusion： The raethod is simple and accurate, can be used for determination of Chlorogenic acid in ErHuaSanCao Mixture.