真菌诱导型植物表达载体pBP的构建

Construction of Fungi Inducible Plant Expression Vector pBP

根据prp1-1启动子序列设计并合成了一对引物,通过PCR扩增的方法从马铃薯基因组中扩增到了病原诱导的prp1-1启动子,并将其克隆到PGEMT-Easy载体上,然后进一步克隆到植物表达载体pBI101.2上,构建了病原诱导型基因表达载体pBP.

According to the reported sequences of inducible expression promoter prp1 - 1, a pair of primers were designed and synthesised. After the fragment of prp1 - 1 promoter was amplified by the method of PCR from the potato, it was inserted into PGEMT- Easy vector. Thereafter, the fragment was cloned into pBI101. 2 expression vector, and a new kind of pathogen - inducible expression vector pBP was constructed.