Effect of Electro-acupuncture on Drug-seeking Behavior of the Heroin Self-administrating Rat

Objective： This present study is to establish a rat model of intravenous heroin self-administration and observe the effect of electro-acupuncture on the heroin seeking behavior of the heroin self-administrating rat. Methods： Six Male Wistar rats were surgically implanted with an intra jugular intravenous catheters under sodium pentobarbital anesthesia, following a minimum recovery of 7 days, the rats were trained by nose-poke response with one daily 4-hour session for 14 consecutive days to self administer heroin （50 μg /kg / per injection）, the session ended after 25 infusions were earned or 4 hours had passed, whichever came first, the reinforcement schedule was fixed ratio 1 （FR1） that the rat received a heroin injection for each nose poke response. And then the rats were given electro-acupuncture treatment daily 30min prior to each session and then were trained with one daily 4-h session for 7 consecutive days. Results： Stable intravenous heroin self-administration behavior of rats was established after 7-8 days＇ drug training, electro-acupuncture given to the stable heroin self-administrating rat 30 min prior to each session could reduce the drug self-administration behavior, namely the total heroin intake in each session was reduced, especially the 1^th hour heroin intake, the initiation of first nose-poke response in each session was relayed, and the haste heroin-seeking behavior also became slower. Conclusions： The above results suggested that the electro-acupuncture had better detoxification effect.

目的:观察电针对海洛因静脉自身给药大鼠觅药行为的影响.方法:采用强化程序为固定比率1(FR=1)的方法训练雄性Wistar大鼠通过鼻触反应进行海洛因静脉自身给药,每天训练4 h,每次鼻触反应后海洛因注射剂量为50 μg/kgg,限量每天25次,连续训练14 d.第15天始,大鼠每天训练前30 min给予电针治疗,然后再进行给药训练,连续治疗7天.结果:大鼠经过7-8 d的自身给药训练后,都开始建立起稳定的静脉自身给药行为,对于已形成稳定自身给药行为的大鼠,每天训练前30 min给予电针干预治疗,可减弱大鼠的静脉自身给药行为,表现为:每个训练期内大鼠的用药总量下降,尤其是第1小时的用药量下降显著;每个训练期内第一个鼻触反应的启动时间延迟;大鼠急促的觅药行为变为缓慢,鼻触反应数亦下降.且这种减弱效应随电针治疗时间的延长而明显.结论:电针具有良好的脱毒效应.