微波固相合成寡糖工艺的初步研究

STUDY ON THE SYNTHETIC CONDITIONS OF BIOACTIVE OLIGOSACCHARIDE BY SOLID STATE REACTIONS UNDER MICROWAVE IRRADIATION

以葡萄糖(C6H12O6.H2O)为原料,以杂多酸为催化剂对生物活性物质寡糖的微波固相合成进行了研究。确定最佳反应条件为:微波功率800瓦,微波辐射时间10min,引发剂水的添加量30%,催化剂酸添加量10%;产物得率为50.6%。经高压液相色谱分析产物,确定微波固相合成物的组成为:葡萄糖49.34%,麦芽糖10.03%,异麦芽糖18.03%,麦芽三糖5.40%,潘糖10.13%,异麦芽三糖5.30%,四糖和五糖占1.61%。

The synthesis of bioactive oligosaccharide with glucose and phosphoric acid as the catalyst by solid state reaction under microwave irradiations was studied. The optimum reaction conditions was found as follows： microwave power 800w ,microwave irradiation time 10 rain, adding 30% water to initiate reaction, adding 10% heteropoly acid A as the catalyst. The yield of product was 50.6%. The product was characterized by HPLC analysis. The constitution of product was established as follows： glucose 49.34%,maltose 10.03%,isomaltose 18.03%, maltotriose5.40%, panose 10.13% , isomaltotriose5.30%, tetrsaccharides and pentasaccharides 1.61%.