高效液相色谱法测定丹参舒心胶囊中丹参酮Ⅱ_A的含量

Determination the Content of Tanshinone Ⅱ_A in Danshen Shuxin Capsule by HPLC

目的:建立以高效液相色谱法测定丹参舒心胶囊中丹参酮ⅡA含量的方法。方法:色谱柱为Shim-PackCLC-ODS,流动相为甲醇-水(15∶5),检测波长为270nm,柱温为40℃,流速为1.5ml/min。结果:丹参酮ⅡA检测浓度在0.05125～0.25625μg/ml范围内线性关系良好(r=0.9996,n=5),平均加样回收率为99.05%(RSD=0.92%)。结论:本方法操作简便、精密度好、结果可靠,可用于丹参舒心胶囊的质量控制。

OBJECTIVE：To establish HPLC method for the determination the centent of tanshinone ⅡA in danshen shuxin capsule. METHODS： The determination was performed on Shim- pack CLC- ODS column, the mobile phase consisted of methanol- water（15：5）, the detection wavelength of 270nm, column temperature at 40℃, a flow rate of 1.5 ml/min and .RESULTS： The calibration curve of tanshinone ⅡA is linear when its concentration range was within 0.05 125-0.25625μg/ ml （r = 0.9 996, n = 5）, the average recovery was 99.05% （RSD = 0.92% ）.CONCLUSION：This method is convenient in operation,precise,reliable in results,and it can be used as quality control for danshen shuxin capsule。