薄层扫描法测定血尿康颗粒中盐酸水苏碱的含量

Determination of Stachydrine Hydrochloride in Xueniaokang Granule by TLC Scanner

目的:建立以薄层扫描法测定血尿康颗粒中盐酸水苏碱含量的方法。方法:采用硅胶G薄层板,展开剂为正丁醇-盐酸-醋酸乙酯(8∶3∶1),显色剂为稀碘化铋钾试液,测定波长为515nm,参比波长为700nm,狭缝大小为6.00mm×0.45mm。结果:盐酸水苏碱点样量在5.06μg～25.32μg范围内与斑点峰面积积分值呈良好的线性关系(r=0.9978),平均加样回收率为96.67%(RSD=2.01%)。结论:本方法简便、灵敏度高、重现性好,可用于血尿康颗粒的含量测定与质量控制。

OBJECTIVE： To establish TLC scanner method for the determination of stachydrine hydrochloride in xueniaokang granule.METHODS： Silica gel G thin layer plate was adopted in the determination with n - butanol - hydrochloric acid- ethyl acetate（8 ： 3 ： 1） used as developer and thin potassium heptaiodobismuthate test solution used as color-developing agent, the detection wavelength was 515nm, the reference wavelength was 700nm and the slit size was 6.00mm × 0.45mm，RESULTS： Good linear correlation with the peak area score of spots achieved when the concentration range of stachydrine hydrochloride was within 5.06μg- 25.32μg（ r = 0.9 978）, the average recovery was 96.67 % （RSD = 2.01% ）. CONCLUSION ： The method was accurate, simple, reliable, sensitive and reproducible, and it can be used for the determination of xueniaokang granule and its quality control。