补充蛋白饮食对饥饿大鼠免疫功能的影响

EFFECT OF SUPPLEMENTATION OF PROTEIN DIET ON IMMUNE FUNC TION IN STARVATION RAT

目的探讨补充蛋白饮食改善饥饿大鼠机体免疫功能.方法青年Wistar鼠,随机分为正常对照组、半饥饿对照组、饥饿对照组、补充蛋白半饥饿组、补充蛋白饥饿组,其中半饥饿组给予正常对照组每日饲料摄入量的50%,饥饿组给予正常对照组每日饲料摄入量的30%,2个补充蛋白组蛋白摄入量与正常对照组相同.分别于1、2周取血清样品测定免疫功能.结果补充蛋白饮食对IgG和IgA水平影响不明显.实验1周时,正常对照组IgM水平[(561±76)mg·L-1]显著高于其他各组(在430～440 mg·L-1之间,P<0.01);实验2周时,补充蛋白半饥饿组[(559±41) mg·L-1]明显高于饥饿对照组[(469±16) mg·L-1]和补充蛋白饥饿组[(473±40) mg·L-1,P<0.05].饥饿2周时,各组C3水平显著降低,但其中补充蛋白半饥饿组[(229±16)mg·L-1]显著高于饥饿对照组[(175±12)mg·L-1]和补充蛋白饥饿组[(180±12)mg·L-1,P<0.01].饥饿可使C4含量不同程度降低,但实验1周时给予补充蛋白的2个组均不降低;至实验2周时,补充蛋白半饥饿组[(64.3±12.1)mg·L-1]显著高于饥饿对照组[(39.2±4.7)mg·L-1,P<0.05].结论给予补充蛋白饮食,在一定程度上可通过防止半饥饿时IgM、C3、C4过分降低来改善机体免疫功能.

Objective To study the effects of supplementation of protein diet on immune function in starvation rat. Methods Male Wistar rats were divided into normal control, semistarvation control, starvation control, semi-starvation with supplementation of protein diet, and starvation with supplementation of protein diet group. Semi - starvation group were given 50% intake of normal control, starvation group were given 30% intake of normal control, and the protein intake of two supplementation groups were equal to that of normal control. Blood samples were collected to examine the immune function 1st week and 2nd week after the experiment. Results There were no significant differences in IgG and IgA among these groups. IgM content was higher in normal control group [（561 ± 76）mg·L^-1]than in the other groups [（430 - 440） mg· L^- 1, P 〈 0.01] in 1 st week, while that in semi - starvation with supplementation group [ （559 ± 41）mg· L^-1] was higher than those in starvation control group, and starvation with supplementation group [ （469± 16）, （473 ± 40） mg· L^-1 respectively, P 〈 0. 05] in 2nd week. C3 level in every group was declined markedly after 2 week. But C3 [ （229 ± 16）mg· L^-1] in semi - starvation with supplementation group was higher than in starvation control, and starvation with supplementation groups [ （ 175±12）, （ 180± 12） mg· L^- 1 respectively, P 〈 0.01]. C4 level decreased to some extent in semi - starvation control, and starvation control groups, but there were not any changes in the two protein supplementation groups in 1st week. After 2 week, C4 level[ （64.3 ± 12.1 mg·L^- ）] in semi - starvation with supplementation group was higher than in starvation control group [ （39.2 ± 4.7） mg· L^- 1, P 〈 0.05]. Conclusion Protein diet may improve the immune function to some extent by increasing the levels of IgM, C3 and C4 in semi - starvation rat.