脑胶质瘤中纤维连接蛋白及其整合素β_1受体的表达

Expression of fibornectin and β_1 integrin receptor in human brain glioma

目的探讨脑胶质瘤中纤维连接蛋白(FN)及其整合素β1受体蛋白(β1integrin)表达的临床病理联系。方法用免疫组化SP法检测56例胶质瘤组织中FN和整合素β1的表达情况,并进行相关临床病理分析。结果(1)胶质瘤组织间质血管壁FN染色随级别增高而增厚(P<0.01),且血管厚度在复发病例组明显高于无复发组(P<0.05)。恶性程度高的Ⅲ级与Ⅳ级胶质瘤组织中还可见基质弥散阳性染色。(2)FN和整合素β1在胶质瘤中阳性表达率分别为30.36%(17/56)和39.29%(22/56),并均随肿瘤级别增加而增高(P<0.05,P<0.01),两者表达存在相关性(rs=0.312,P<0.05)。复发病例FN和整合素β1阳性表达率(68.42%,57.89%)均高于无复发组(10.81%,29.73%)(P<0.01,P<0.05)。结论FN及其整合素β1受体在胶质瘤组织中的高表达促进了胶质瘤的恶性进展,两者可能成为有价值的判断胶质瘤恶性程度及预后的指标。

Purpose To study the relationship between the expression of fibronectin （FN） and β1 integrin receptor and the clinical pathology of human brain glioma （BG）. Methods Immunohistochemistry was used to detect the protein expression of FN and β1 integrin in 56 human glioma specimens. Correlative clinicopathologic analysis was performed in this study. Results With the increase of malignant grading, the expression of FN grew stronger in the vessel walls of BG （P〈0.01）. It was also well correlated with recurrence （P〈0.05）. The diffuse positive stain could also be seen in the stroma of grade Ⅲ and grade Ⅳ tumors. The positive rates of FN and β1 integrin in the tumor cells were increased with malignant grading （P〈0.05 ,P〈0.01）, which was 30.36% （17/56） and 39.29% （22/56） respectively. Significant positive correlation was observed between their expression （rs=0.312, P〈0.05）. The expression of FN and β1 integrin was higher in the recurrence group （68.42% and 57.89% respectively） than that in the non recurrence group （10. 81% and 29.73% respectively, P〈0.01 ,P〈0.05 ）. Conclusions The high expression of FN and β1 integrin may play the important roles in the malignant progressions of BG and can be served as valuable indicators of malignancy and prognosis of BG.