螺旋藻抗运动性疲劳作用的实验

Effect of spirulina against exercise-induced fatigue

目的:观察螺旋藻对大鼠力竭运动时间及血清中乳酸脱氢酶、磷酸肌酸激酶、谷草转氨酶活性和丙二醛浓度的影响。方法:实验于2003-09/2004-01在广西医科大学生理实验室完成。30只SD雄性大鼠随机分为安静组、力竭运动组、螺旋藻力竭运动组,每组10只。安静组、力竭运动组喂普通饲料,螺旋藻力竭运动组在饲料中加入15%螺旋藻干粉喂养,共喂养1个月。力竭运动组、螺旋藻力竭运动组大鼠在跑台上进行下坡运动至力竭,跑台速度16m/min,坡度为-16°,力竭标准为动物已跟不上预定速度,经电刺激尾巴仍不能继续往前跑。力竭运动组、螺旋藻力竭运动组大鼠力竭后即刻腹腔麻醉断头处死,同时亦将安静组麻醉后处死,颈动脉采血,分离血清,检测各组大鼠血清乳酸脱氢酶、磷酸肌酸激酶、谷草转氨酶活性、丙二醛的水平,评估机体运动损伤的情况。结果:30只大鼠均进入结果分析。①螺旋藻力竭运动组力竭运动时间明显少于力竭运动组(81.4±13.7),(72.8±15.3)min,(t=2.12,P=0.048)。②力竭运动组血清乳酸脱氢酶、磷酸肌酸激酶、谷草转氨酶活性、丙二醛浓度明显高于安静组(86.6±4.0),(42.6±4.6)μkat/L;(6.62±0.38),(4.82±0.31)μkat/L;(7.90±0.48),(5.84±0.72)μkat/L;(6.16±0.36),(4.47±0.15)μmol/L,(q=29.70,16.09,11.50,17.49,P=0.000),螺旋藻力竭运动组上述指标明显低于力竭运动组(53.6±5.3)μkat/L,(5.85±0.35)μkat/L,(6.90±0.45)μkat/L,(4.64±0.29)μmol/L,(q=19.22,6.89,5.56,15.73,P<0.01)。结论:螺旋藻可减轻力竭运动后代谢引起的氧自由基损伤,保护细胞膜结构的稳定,调节渗透压平衡,防止过氧化损伤,从而起到抗运动性疲劳的作用。

AIM： To observe the effect of spirulina on the time of exhaustive exercise, activities of lactate dehydrogenase,creatine phosphate kinase and glutamie- oxaloacetic transaminase,and the concentration of malondialdehyde in serum of rats. METHODS： The experiment was carried out in the physiological laboratory of Guangxi Medical University between September 2003 and January 2004. Thirty male adult SD rats were randomly divided into quiet group （n=10）, exhaustive exercise group （n=10）, spirulina exhaustive exercise group （n=10）. Rats in the quiet group and exhaustive exercise group were fed with common feed, and 15% spirulina dry powder was added into the feed in the spirulina exhaustive exercise group, all the rats were fed for 1 month. Rats in the exhaustive exercise group and spirulina exhaustive exercise group were forced to keep running on the treadmill with down slope till exhaustion, the velocity of treadmill was 16 m per minute, the gradient was -16°; The criteria of exhaustion was that the rats could keep its pace with the fixed velocity, and they still could not go on running after electrostimulation at tail; The rats in the two exhaustive exercise groups were killed immediately after exhaustion, and those in the quite group were also killed at the same time, blood samples were taken from carotid artery, and then serum was isolated, the activities of lactate dehydrogenase, creatine phosphate kinase and glutamic-oxaloacetic transaminase, and the concentration of malondialdehyde in serum were detected to evaluate the severity of exercise injury. RESULTS： All the 30 rats were involved in the analysis of results. ① The time of exhaustive exercise was obviously shorter in the spirulina exhaustive exercise group than in the exhaustive exercise group [（81.4±13.7）, （72.8±15.3） minutes, t=2.12, P=0.048].② The serum activities of lactate dehydrogenase, creatine phosphate kinase and glutamic-oxaloacetic transaminase, and the concentration of malondialdehyde were obviously higher in the exhaustive exercise group than in the quiet group [（86.6±4.0）, （42.6±4.6） μkat/L; （6.62 ±0.38）, （4.82 ±0.31） μkat/L; （7.90±0.48）, （5.84±0.72） μkat/L; （6.16 ±0.36）, （4.47 ±0.15）μmol/L, q=29.70, 16.09, 11.50, 17.49, P=0.000], but markedly lower in the spirulina exhaustive exercise group than in the exhaustive exercise group [（53.6±5.3） μkat/L, （5.85 ±0.35） μkat/L, （6.90±0.45）μkat/L, （4.64 ±0.29） μmol/L, q=19.22, 6.89, 556, 15.73, P 〈 0.01]. CONCLUSION： Spirulina can relieve oxygen-derived free radicals injury caused by metabolism after exhaustive exercise, protect the stable structure of cell membrane, adjust the balance of osmotic pressure, prevent peroxidative injury, and then plays a role against exercise- induced fatigue.