期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

胚胎细胞MAD2基因表达对染色体分离的影响 被引量：1

Effects of MAD2 expression in embryonic cells on chromosome segregation
下载PDF
导出
摘要 目的探讨MAD2基因表达对染色体分离的影响。方法用定量RT-PCR比较RNA干扰前后的胚胎细胞内源性基因MAD2 mRNA表达水平,同时对染色体数目异常率进行比较分析。结果干扰后MAD2基因mRNA拷贝数/GAPDH基因mRNA拷贝数的均值为干扰前的20%,染色体数目异常率均值由干扰前的4.702%上升到28.498%。经统计学分析,RNA干扰后MAD2基因mRNA水平显著下降,染色体数目异常率显著增高。结论MAD2基因表达的降低会导致染色体分离的异常,本研究为染色体分离相关蛋白功能研究建立了新的研究手段和实验评价体系。 Objective To investigate the effects of MAD2 expression in embryonic cells on chromosome segregation. Methods Endogenous MAD2 mRNA level was compared by using quantitative RT-PCR and the rate of chromosome number aberration were evaluated in embryonic cells before and after RNA interference. Results The ratio of MAD2 mRNA/GAPDH mRNA after RNA interference decreased to 20% of the ratio before RNA interference （P 〈 0. 01 ）. The rate of chromosome number aberration increased from 4. 702% before RNA interference to 28. 498% after interference. Conclusion The decreased MAD2 expression might lead to abnormality of chromosome segregation.
作者 施琼 翁亚光 蔡燕 刘青松 刘子杰
机构地区 重庆医科大学临床检验诊断学实验室
出处 《第三军医大学学报》 CAS CSCD 北大核心 2005年第15期1544-1547,共4页 Journal of Third Military Medical University
基金 国家自然科学基金资助面上项目(30371485)~~
关键词 MAD2基因 RNA干扰 定量RT—PCR MAD2 RNA interference quantitive RT-PCR
分类号 R394.2 [医药卫生—医学遗传学] Q343.2 [生物学—遗传学]
  • 相关文献

参考文献10

  • 1Rieder C L, Cole R W, Khodjakov A, et al. The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores[J]. J Cell Biol,1995, 130(4): 941 -948.
  • 2DeAntoni A, Sala V, Musacchio A. Explaining the oligomeriz ation properties of the spindle assembly checkpoint protein Mad2[J]. Philos Trans R Soc Lond B Biol Sci, 2005, 360(1455): 637 -648.
  • 3Kops G J, Foltz DR, Cleveland D W. Lethality to human cancer cells through massive chromosome loss by inhibition of the mitotic checkpoint[J]. Proc Natl Acad Sci U S A, 2004, 101(23): 8699 -8704.
  • 4Homer H A, McDougall A, Levasseur M, et al. Restaging the spindle assembly checkpoint in female mammalian meiosis I [ J ]. Cell Cycle,2005, 4 (5): 650 - 653.
  • 5Steuerwald N, Cohen J, Herrera R J, et al. Association between spindle assembly checkpoint expression and maternal age in human oocytes[J]. Mol Hum Reprod, 2001, 7(1): 49 -55.
  • 6Qin Z L, Zhao P, Zhang X L, et al. Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells [ J ]. Biochem Biophys Res Commun, 2004, 324(4): 1186-1193.
  • 7Machuy N, Thiede B, Rajalingam K, et al. A global approach combining proteome analysis and phenotypic screening with RNA interference yields novel apoptosis regulators [ J ]. Mol Cell Proteomics, 2005, 4 (1): 44-55.
  • 8Johnson V L, Scott M I, Holt S V, et al. Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression[J]. J Cell Sci, 2004, 117(Pt 8): 1577 - 1589.
  • 9Schaar B T, Chan G K, Maddox P, et al. CENP-E function at kinetochores is essential for chromosome alignment [J]. J Cell Biol, 1997,139(6): 1373 - 1382.
  • 10Meraldi P, Draviam V M, Sorger P K. Timing and checkpoints in the regulation of mitotic progression [ J ]. Dev Cell, 2004, 7 ( 1 ): 45 -60.

同被引文献13

  • 1何俊琳,曹波,王应雄.BGC823和A549细胞染色体着丝粒点变异[J].遗传,2005,27(6):877-881. 被引量：13
  • 2Sugimoto K, Fukuda R, Himeno M. Centromere/kinetochroe localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein[J]. Cell Struct Funct, 2000, 25(4) : 253 -261.
  • 3Vig B K, Steraes K L. Centromeres without kinetochore proteins. Another mechanism for origin of aneuploidy in neoplasia[ J]. Cancer Gen- et Cytogenet, 1991, 51(2): 269-272.
  • 4Nakagome Y, Abe T, Misawa S, et al. The "loss" of centromeres from chromosomes of aged women[ J]. Am J Med Genet, 1984, 36 (2) : 398 - 404.
  • 5Ganem N J, Godinho S A, Pellman D. A mechanism linking extra centrosomes to chromosomal instability [ J ]. Nature, 2009, 460 ( 7252 ) : 278 - 282.
  • 6He J, Liu X, Ding Y, et al. An improved method for staining kinetochores of human chromosomes [ J ]. Indian J Exp Biol, 2009, 47 (5) : 376 - 378.
  • 7Santaguida S, Musacchio A. The life and miracles of kinetochores [ J].EMBO J, 2009, 28 ( 17 ) : 2511 - 2531.
  • 8Palframan W J, Meehl J B, Jaspersen S L, et al. Anaphase inactivation of the spindle checkpoint [ J ]. Science, 2006, 313 (5787) : 680 - 684.
  • 9Bardhan A. Complex regulation of sister kinetochore orientation in meiosis-I [J]. J Biosei, 2010, 35(3): 485-495.
  • 10Mukhopadhyay D, Dasso M. The fate of metaphase kinetochores is weighed in the balance of SUMOylation during S phase[ J]. Cell Cycle, 2010, 9(16) : 3194 -3201.

引证文献1

二级引证文献4

第三军医大学学报
2005年 第15期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部