模拟微重力环境促进人间充质干细胞体外高效增殖

High-performance proliferation of hMSCs in the mimic microgravity environment

目的观察人骨髓间充质干细胞(hum an m esenchym al stem cells,hMSCs)在模拟微重力环境中的体外增殖情况。方法在旋转细胞培养系统(rotary cell cu lture system,RCCS)模拟的微重力环境中,用Cytodex-3微载体技术培养hM-SCs,通过相差显微镜、细胞计数、MTT法、CCK-8(cell counting K it-8)观察检测hMSCs的增殖状况。结果试验组hMSCs较平皿培养组延长指数生长期提高2倍,细胞增殖速度提高近2倍,培养密度增加15.8倍。结论模拟微重力环境能促进hMSCs的体外增殖速度,利于体外规模化扩增组织工程的种子细胞。

Objective To observe the proliferation of human bone marrow MSCs cultured under the mimic microgravity condition in vitro. Methods The hMSCs were cultured in the mimic microgravity environment founded by rotary cell culture system and Cytodex-3 microcarrier. The proliferation of hMSCs were observed by phase contrast microscope and detected by cell counting, MrFr assay and CCK-8 assay. Results The exponential growth period of hMSCs extended to double, the speed of proliferation increased to nearly two times, and the density of hMSCs in the medium increased by 15.8 times as compared with the plate-culture method. Conclusion The mimic microgravity condition and microcarrier benefit the proliferation of hMSCs in vitro, and also fit to produce a large quantity of seeding cells for tissue engineering.