芦丁对绿豆幼苗营养生长的影响及其与IAA的相互作用(英文)

Effects of Rutin on Vegetative Growth of Mung Bean (Vigna radiata)Seedlings and Its Interaction with Indoleacetic Acid

观察了植物体内的天然黄酮芦丁和吲哚乙酸(IAA)对绿豆幼苗营养生长的影响并测定胚轴中的芦丁和IAA含量.光照条件下芦丁(60μg/mL以下)处理对绿豆幼苗生长有一定促进作用,表现为胚轴和主根伸长加快、侧根数目增多、鲜重或干重增加;而光照条件下更高浓度芦丁(80μg/mL以上)处理及黑暗条件下芦丁(20～100μg/mL)处理对绿豆幼苗生长有抑制作用.当培养基中的芦丁浓度为60～80 μg/mL时,光照下的幼苗比暗处理的幼苗在胚轴中积累更多的芦丁;而芦丁浓度为40μg/mL以下和接近100μg/mL时幼苗在光照下累积的芦丁较暗处理的幼苗更少.0.1μg/mL以上的IAA促进芦丁的累积而进一步抑制幼苗胚轴和主根的伸长.当培养基中含有40 μg/mL的芦丁和0.5μg/mL的IAA时,胚轴中累积的芦丁达到高峰.芦丁降低黄化幼苗内源性IAA在胚轴中的累积,并抑制幼苗对IAA的吸收.

Rutin is one of the natural flavonoids in plants. Effects of turin and indoleacetic acid （IAA） on vegetative growth of mung bean seedlings were studied and the contents of rutin and IAA in the hypocotyls of the seedlings were measured. Mung bean seedlings absorbed rutin through roots, grew more vigorously under light at lower rutin concentrations （20-60μg/mL） with longer shoot axis （epicotyl＋hypocotyl） and primary root and more lateral roots. The seedlings exhibited inhibition of elongation of the hypocotyls and radicles when grown in the dark at rutin concentrations of 20-100 μg/mL or under light at rutin concentrations of 80-100 μg/mL. Seedlings under light absorbed more rutin than those in the dark at rutin concentrations of 60-80 μg/mL and absorbed less rutin at concentrations lower than 40 μg/mL or near 100 μg/mL, IAA （〉0.1 μg/mL） treatment promoted rutin accumulation and inhibited elongation of shoot axis and primary roots. The rutin content in hypocotyls reached the highest level when IAA （0.5 μg/mL） and rutin （40μg/mL） were added to the medium. Rutin repressed endogenous IAA accumulation in hypocotyls of the etiolated seedlings and inhibited absorption of exogenous IAA from media to seedlings.