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迷走神经切除对大鼠胃内UCP_2 mRNA表达及胃酸分泌的影响 被引量:2

EFFECTS OF VAGOTOMY ON UCP_2 mRNA EXPRESSION AND GASTRIC ACID SECRETION IN RATS
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摘要 目的:观察整体大鼠胃酸分泌与ATP水平之间的关系及迷走神经对解偶联蛋白2(UCP2)mRNA表达的调节。方法:制备大鼠高选择性迷走神经切断模型。采用滴定法检测大鼠胃酸酸度,荧光测定法检测胃体组织内ATP含量,应用Northernblot法检测分析UCP2mRNA表达。结果:迷走神经切断后24h,大鼠胃酸酸度显著降低,胃体组织内ATP含量下降。与假手术组相比,胃体组织UCP2mRNA表达显著增加。结论:迷走神经切断后24h大鼠胃体组织ATP含量下降;迷走神经下调胃体组织UCP2mRNA表达。结果提示大鼠迷走神经可能通过抑制UCP2mRNA表达,提高ATP含量,为质子泵泌酸提供能量来源。 Aim: The relationship between gastric acid secretion and ATP level, and regulation of uncoupling protein2(UCP2) mRNA expression by vagus nerve were studied in vagotomied rats. Methods: With the high selective vagotomy model, the gastric acidity was titrated to pH 7.0 with 0.1 mol/L NaOH solution and ATP contents were quantified by using fluoremetry. The expression of UCP2 mRNA was observed by using Northern blot in stomach of rats. Results: Both of gastric acidity and ATP contents in stomach body decreased significantly at 24 h after vagotomy. The expression of UCP2 mRNA was markedly increased as compared with sham operation group. Conclusion: ATP contents decreased and vagus nerve down-regulates expression of UCP2 mRNA in stomach corpus in vagotomied rats. The results indicates that vagus nerve could underlay the gastric acidity by inhibiting expression of UCP2 mRNA and increasing ATP contents in rats
出处 《中国应用生理学杂志》 CAS CSCD 北大核心 2005年第3期290-292,共3页 Chinese Journal of Applied Physiology
基金 教育部留学回国人员科研启动基金资助(200314) 辽宁省教育厅科技攻关计划项目(202203243)
关键词 解偶联蛋白2 胃迷走神经切断术 胃酸分泌 ATP uncoupling protein 2 vagotomy gastric acid secretion ATP
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参考文献8

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同被引文献13

  • 1叶翠飞,白容,刘汇波,郭德玉,李斌,李林.双侧颈总动脉结扎对大鼠学习记忆相关脑区血流量的影响[J].中国实验动物学报,1999,7(1):23-25. 被引量:13
  • 2Xue-Ying Zhang Hong-Bin Ai Xi-Yun Cui.Effects of nuclei ambiguus and dorsal motor nuclei of vagus on gastric H^+ and HCO_3^- secretion in rats[J].World Journal of Gastroenterology,2006,12(20):3271-3274. 被引量:5
  • 3王林婕,徐莉,孙一耕,艾洪滨.夹闭大鼠双侧颈总动脉对胃运动的影响[J].生物医学工程研究,2007,26(1):59-61. 被引量:2
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