高压液相荧光检测法检测血浆同型半胱氨酸

DETERMINATION OF HOMOCYSTEINE LEVEL IN PLASMA BY HIGH PERFORMANCE LIQUIDS CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

目的:建立高压液相荧光检测法测定血浆中同型半胱氨酸(homocysteine,Hcy)的方法.方法:应用Symmetry ShieldTMRP18色谱柱,0.08 mol/L醋酸钠1%甲醇为流动相,与巯基特异结合的荧光物质SBD-F衍生巯基来检测血浆中的同型半胱氨酸浓度.结果:该法的平均回收率为95.8～100.8%,相对标准差为1.2～2.0%.结论:本检测法方法准确,可用于实验室样品的测定.

Aim： To establish a method for detection of plasma total homocysteine with HPLC. Methods： The chromatography analysis was carried out using a Symmetry ShieldTMRP18. The mobile phase was sodium acetate（0.08 mol/L） and methanol（ 1% ） and we utilized a HPLC system with fluorescence detection of plasma homocysteine derivatized from reaction with 7-fluorobenzo-2-oxa-1,3-diazole-4- sulphonate（SBD-F）. Results： The average recoveries were 95.8- 100.8% and the relative standard deviations were 1.2 - 2.0%. Conclution： The results showed it to be a rapid and accurate method for the determination of homocysteine level in plasma.