摘要
目的:建立高压液相荧光检测法测定血浆中同型半胱氨酸(homocysteine,Hcy)的方法.方法:应用Symmetry ShieldTMRP18色谱柱,0.08 mol/L醋酸钠1%甲醇为流动相,与巯基特异结合的荧光物质SBD-F衍生巯基来检测血浆中的同型半胱氨酸浓度.结果:该法的平均回收率为95.8~100.8%,相对标准差为1.2~2.0%.结论:本检测法方法准确,可用于实验室样品的测定.
Aim: To establish a method for detection of plasma total homocysteine with HPLC. Methods: The chromatography analysis was carried out using a Symmetry ShieldTMRP18. The mobile phase was sodium acetate(0.08 mol/L) and methanol( 1% ) and we utilized a HPLC system with fluorescence detection of plasma homocysteine derivatized from reaction with 7-fluorobenzo-2-oxa-1,3-diazole-4- sulphonate(SBD-F). Results: The average recoveries were 95.8- 100.8% and the relative standard deviations were 1.2 - 2.0%. Conclution: The results showed it to be a rapid and accurate method for the determination of homocysteine level in plasma.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2005年第3期353-355,共3页
Chinese Journal of Applied Physiology
基金
国家重点基础研究发展规划项目(001CB510207)
国家自然科学基金资助课题(30370586)