应用组织微阵列技术研究MMP-9与TIMP-1在卵巢上皮性肿瘤中的表达及临床意义

Expression and Clinical Significance of MMP-9and TIMP-1in Epithelial Ovarian Tumor Using Tissue Microarray Technique

目的研究基质金属蛋白酶-9(MMP-9)及其组织基质金属蛋白抑制剂-1(TIMP-1)在卵巢上皮性肿瘤中的表达及其临床意义。方法应用组织微阵列技术结合免疫组化(S-P法)检测94例卵巢上皮性肿瘤组织中MMP-9和TIMP-1的表达。结果卵巢癌组织和卵巢交界性肿瘤组织中MMP-9阳性表达率明显高于卵巢良性肿瘤(P<0.01);卵巢癌组织中TIMP-1阳性表达率明显高于卵巢良性肿瘤(P<0.01);MMP-9和TIMP-1表达与患者年龄、肿瘤大小和卵巢组织学类型均无明显相关性;在卵巢癌的病理分级中,MMP-9/TIMP-1比值随卵巢上皮性肿瘤恶性程度的增加明显增大。结论MMP-9和TIMP-1在卵巢上皮性肿瘤的演进发展中起重要作用,二者可作为卵巢上皮性肿瘤恶性化的分子指标之一。组织微阵列是检测多样本组织的一种高效、低耗、可比性强的分子病理学研究工具。

Objective To study the expression of Matrix Metalloproteinase-9 （MMP-9） and Tissue inhibitor of metalloproternase-1 （TIMP-1） in epithelial ovarian tumor and their clinical significance. Methods The expressions of MMP-9 and TIMP-1 in 94 cases of epithelial ovarian tumor were determined by tissue microarray combined with immunohistochemistry. Results The positive rates of MMP-9 in borderline and ovarian adenocarcinoma tissue were significantly higher than that in benign tumor tissue（P〈0. 01 ）. The positive rate of TIMP-1 in ovarian adenocarcinoma tissue was also significantly higher than that in benign tumor tissue（P〈0.01）. The expressions of MMP-9 and TIMP-1 weren＇t related to patients＇ age, tumors＂ size and histologic types,but with histologic grade, the proportionality of MMP-9/ TIMP-1 increased obviously with growing malignant potential of ovarian adenocarcinoma. Conclusion The expression of MMP-9 and TIMP-1 may play an important role in the development of malignant epithelial ovarian tumor, which may serves as a marker for diagnosis in borderline and ovarian adenocarcinoma. Tissue microarray is an efficient , low consume and strong comparability technique in study of human pathology.