摘要
针对部分杂交稻在生产上不抗病的问题,通过传统选育方法与现代基因工程技术的结合,将转溶菌酶基因水稻中花9号(粳稻)与杂交稻亲本MH63(籼稻)进行杂交和多次回交,以期快速筛选出持久高抗的优良株系,进而配制新的杂交稻组合,获得既抗稻瘟病且农艺性状优良的杂交稻亲本新品系.试验结果表明,转育后代对叶瘟和穗颈瘟的抗性均明显优于轮回亲本,叶瘟的病情指数降低了39.39,穗颈瘟的发病率降低了10.21%.经病圃筛选和室内分子检测,证明转育后代已携带有溶菌酶基因.现已得到167个综合抗性好且表型趋向于轮回亲本的单株.
Traditional breeding method and modem genetic obtain new strains of high resistance and create new hybrid engineering technology are combined in order to efficiently rice with fine agronomic characters and strong resistance to rice blast germ. By crossing and continual back crossing, lysozyme gene has been transferred from transgenic rice Zhonghua9 （ZH9（R））——japonica rice to hybrid rice parents Minghui63（MH63）——indica rice. That has been proved by way of filtration of resistance and PCR analysis. The resistance of transferring generations are much better than their indica type rice parent. Disease index of leaf blast and loss percent of spike blast are respectively decreased by 39.39 and 10.21%. Now 167 offspring has been attained.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第4期409-411,共3页
Journal of Hunan Agricultural University(Natural Sciences)
基金
教育部科学研究重点项目(204101)
湖南省教育厅科学研究重点项目(03A016)
湖南农业大学稳定人才基金项目(05WD13)
关键词
溶菌酶基因
转育
粳稻
籼稻
稻瘟病
lysozyme gene
transfer
japonica rice
indica rice
rice blast
