用荧光物质浸泡标记胭脂鱼仔、稚鱼耳石

Otolith Marking of Larval and Juvenile Chinese Sucker with Fluorescent Substances

用茜素络合物和盐酸四环素溶液分别对胭脂鱼(Myxocyprinus asiaticus)仔、稚鱼浸泡标记,结果表明,100～200mg/L的茜素络合物溶液浸泡24h对胭脂鱼仔、稚鱼耳石有很好的标记效果.相同浸泡浓度下,随着日龄的增长,耳石上的荧光反应强度降低.三对耳石中,微耳石和矢耳石对茜素络合物较敏感,星耳石敏感性则较低.盐酸四环素溶液对仔、稚鱼耳石的标记效果很差,浸泡液浓度为100mg/L和120mg/L时,仅能在微耳石上检测到较弱的荧光标记,而150mg/L及以上浓度的浸泡液对稚鱼有较高的致死作用.因此,茜素络合物是对胭脂鱼早期鱼苗进行化学标记比较合适的荧光物质,而盐酸四环素不适于标记该鱼的耳石.在对标记鱼进行荧光检测时,矢耳石和微耳石是适合的材料.

Larval and juvenile Chinese Sucker（ Myxocyprinus asiaticus ） were immersed in 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L, 250 mg/L alizarin complexone （ALC） and 50 mg/L, 100 mg/L, 120 mg/L, 150 mg/L, 200 mg/L, 250 mg/L tetracyclin-hydrochlorid （TCHC） solutions for 24 h, respectively, Otoliths of larvae and juveniles were removed and examined under the fluorescent microscope after being reared for a period of time, Fluorescent zones were observed in otoliths of larvae and juveniles immersed in different ALC solutions, and the marks were very evident in 100 mg/L, 150 mg/L and 200 mg/L groups. The marking rate was very low in 50 mg/L group, and the mortality was very high in 250 mg/L group. At the same solution concentration, the fluorescent reaction intensity in otoliths decreased with increasing of fish age, In the three pairs of otoliths, the marking in sagitta and lapiUus was very strong, while the marking in asteriscus was very poor, Fluorescent zones were also observed in otoliths of larvae and juveniles immersed in 100 mg/L and 120 mg/L TCHC solutions, but the marking rates were relatively low and the marks were very weak, When immersed in 150 mg/L,200 mg/L and 250 mg/L TCHC solutions, the mortality of larvae and juveniles was relatively high, So ALC is a suitable substance for otolith marking of Chinese Sucker fry, while TCHC is unsuitable.